Rabbit Anti-Factor VIII Polyclonal Antibody #abs130093

abs130093-50ug

1-2 Weeks

201

abs130093-100ug

1-2 Weeks

301

Description

Factor VIII, an important coagulation factor, collaborates with calcium and phospholipid to serve as a cofactor for factor IXa during the conversion of factor X to its activated form, factor Xa. This protein, belonging to the multicopper oxidase family, plays a crucial role in blood clotting. Additionally, it interacts with von Willebrand factor (vWF), a key component in hemostasis. The binding of vWF to factor VIII is vital for stabilizing its presence in the circulation.

Other names

Factor VIII, also known as antihemophilic factor or coagulation factor VIII, is an essential protein involved in blood clotting. It is encoded by the F8 gene and has different isoforms, including F8b and F8c. Factor VIII plays a crucial role in the coagulation pathway and is required for the formation of a stable blood clot.
Deficiency or malfunction of factor VIII results in a bleeding disorder called Hemophilia A, also known as classic hemophilia. This inherited condition can cause prolonged or spontaneous bleeding, particularly in the joints and muscles.
Factor VIII, often referred to as FVIII or factorVIII, is composed of two chains - a heavy chain and a light chain. The light chain is also known as Factor VIIIa light chain. It is involved in the procoagulant activity of factor VIII.
The F8 gene is located on the X chromosome. Mutations in this gene can lead to a decrease or complete absence of functional factor VIII, causing Hemophilia A. Several genetic variants and isoforms of factor VIII have been identified, such as DXS1253E.
Research and advancements in understanding factor VIII have led to the development of treatments for hemophilia A, including replacement therapies with recombinant factor VIII or plasma-derived factor VIII concentrates.
In summary, antihemophilic factor (factor VIII) is a critical component of blood clotting and is responsible for preventing excessive bleeding. Its deficiency results in Hemophilia A, a genetic bleeding disorder. The identification of different isoforms and genetic variants of factor VIII has contributed to improved treatments for this condition.

Source

Rabbit

Specificity

The detection of Factor VIII antibody is useful in measuring the levels of total endogenous Factor VIII. By detecting this antibody, it is possible to determine the overall amount of Factor VIII present within the body.

Species Reactivity

Human;Mouse

Predictive reaction species

Rabbit;Dog;

Application

The recommended dilution ratios for WB is 1:500~1:3000, for IHC it is 1:50~1:200, for IF/ICC it is 1:100-1:500 and for ELISA (peptide) it is 1:20000-1:40000. To generate a similar content, we can say that for various applications such as western blotting, immunohistochemistry, immunofluorescence and ELISA (peptide), different dilution ratios should be used. For WB, the recommended dilution ratio is between 1:500 to 1:3000, for IHC it is between 1:50 to 1:200, for IF/ICC it is between 1:100 to 1:500 and for ELISA (peptide) it is between 1:20000 to 1:40000.

Immunogen

A human Factor VIII-derived peptide has been synthesized, exhibiting strong similarity to the original protein. The peptide's chemical structure closely resembles its natural counterpart, making it a promising candidate for use in therapeutic applications. Thanks to advancements in peptide synthesis techniques, researchers can now create custom peptides with high precision, paving the way for more effective treatments for various medical conditions.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, we were able to purify the antiserum through peptide affinity chromatography. The purified antiserum was obtained as a result of this process.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the freshness and quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to repeated cycles of freezing and thawing. Proper storage conditions play a significant role in preserving the product's integrity and preventing any deterioration over time.

Storage buffer

This product contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain stability, it should be stored at -20°C and is guaranteed to remain stable for 12 months from the date of receipt. It is important to note that this information is based on the original text.

Target

Background

When factor VIII is combined with calcium and phospholipid, it serves as an important cofactor for the enzyme F9, also known as factor IXa. This partnership plays a key role in the conversion of factor X, or F10, to its activated form, factor Xa. In essence, factor VIII acts as a facilitator, enabling the enzymatic reaction to occur more efficiently and effectively. Without factor VIII, the activation of factor Xa would proceed at a much slower pace, if at all.

Posttranslational modification

Sulfation on Tyr-1699 is essential for binding vWF.

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;

UniPort

P00451