ERK1/2 Antibody #abs130092

abs130092-50ug

In Stock

201

abs130092-100ug

In Stock

301

Description

ERK1 p42 MAP kinase is a crucial regulator of cell growth and differentiation. It plays a critical role in responding to various external cues, such as growth factors, neurotrophic factors, cytokines, hormones, and neurotransmitters. Activated by these signals, ERK1 coordinates and directs cellular processes related to growth and differentiation.
On the other hand, ERK2 p44 MAP kinase, another significant player in cell regulation, also governs cell growth and differentiation. It acts as a central hub where multiple biochemical signals integrate. This integration allows ERK2 to engage in diverse cellular processes like proliferation, differentiation, transcription regulation, and development.
To sum up, both ERK1 p42 MAP kinase and ERK2 p44 MAP kinase play critical roles in cell growth and differentiation. ERK1 responds to a wide range of extracellular signals, while ERK2 acts as an integration point for various biochemical cues. Together, they orchestrate and control essential cellular processes for proper cell function and development.

Other names

The protein ERK1, also known as extracellular signal-regulated kinase 1 or MAP kinase 3, is encoded by the ERK1 gene (HGNC6877). It is also referred to as ERK-1, ERK1, ERK1-1, ERT2, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, MAPK, MAPK1, Mapk3, MGC20180, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, p44 ERK1, p44-MAPK, P44ERK1, Protein Kinase Mitogen Activated 3, etc.
Similarly, the protein ERK2, also known as extracellular signal-regulated kinase 2 or MAP kinase 1, is encoded by the ERK2 gene. It is also referred to as ERK-2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK, MAPK1, Mapk2, Mitogen-activated protein kinase 1, MK01_HUMAN, P38, P40, P41, p42-MAPK, P42MAPK, PRKM1, PRKM2, and protein tyrosine kinase ERK2.
These proteins are important components of the mitogen-activated protein kinase (MAPK) signaling pathway and play crucial roles in various cellular processes, including cell proliferation, differentiation, survival, and apoptosis. They are activated by extracellular signals and regulate the activity of several downstream targets, leading to the modulation of gene expression and cellular responses.
In summary, ERK1 and ERK2 are highly similar proteins that are involved in the regulation of various cellular processes through the MAPK signaling pathway. They have multiple names and are referred to by different aliases based on their functions and characteristics.

Source

Rabbit

Specificity

The detection of total ERK1/2 is achieved by the ERK1/2 antibody, which specifically recognizes the endogenous levels of ERK1/2.

Species Reactivity

Human;Mouse;Rat;Pig;Zebrafish;Bovine;Horse;Sheep;Dog;Monkey;Fish

Application

The recommended dilution for WB is between 1:1000 to 1:5000, while for IHC it is between 1:100 to 1:500. For IF, a dilution of 1:200 is recommended, whereas for ELISA (peptide), a dilution of 1:20000 to 1:40000 is suitable. To ensure optimal results, it is essential to use the recommended dilution for each application. The dilution range may vary depending on the specific experimental conditions, but it is crucial to maintain consistency and accuracy in the dilution process. By following the recommended dilution guidelines, researchers can obtain reliable and reproducible results.

Immunogen

A synthesized peptide derived from human ERK1/2.

Properties

Concentration

1mg/ml

Purification

Peptide affinity chromatography was utilized in purifying the antiserum, and this was accomplished using SulfoLink™ Coupling Resin.

Clonality

Polyclonal Antibody

Stability & Storage

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

PBS, pH 7.4,50% glycerol.

Target

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Posttranslational modification

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Extracellular region or secreted;Golgi apparatus;Mitochondrion;Nucleus;Plasma Membrane;

UniPort

P27361/P28482