Rabbit Anti-VEGFR2 Polyclonal Antibody #abs120548

abs120548-50ul

In Stock

201

abs120548-100ul

In Stock

301

Other names

Fetal Liver Kinase 1 (FLK-1), also known as VEGF Receptor 2 or CD309, is a type III receptor tyrosine kinase. It is also referred to as CD309 antigen, KDR, Ly73, or VGFR2_HUMAN. FLK-1 plays a crucial role in angiogenesis and vascular development. As a protein tyrosine kinase receptor, FLK-1 is responsible for the activation of downstream signaling pathways in response to binding with vascular endothelial growth factors (VEGFs). These growth factors stimulate the proliferation, migration, and survival of endothelial cells.
FLK-1, characterized by its kinase insert domain, functions as a receptor for VEGFs. The receptor's activation leads to the activation of several intracellular signaling cascades, ultimately promoting the growth and development of blood vessels. It is widely expressed in vascular endothelial cells and is essential for the formation of blood vessels during embryonic development and wound healing in adults.
FLK-1, or VEGFR-2, is an important target for therapeutic interventions related to angiogenesis inhibition in cancer treatment. Understanding the role of FLK-1 and its signaling pathways may provide valuable insights into the development of novel therapies for diseases associated with abnormal angiogenesis, such as cancer and cardiovascular disorders.

Source

Rabbit

Specificity

VEGFR2

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;Horse;Sheep;

Application

For ELISA, a dilution of 1:500-1000 is recommended, while for IHC-P and IHC-F, a dilution of 1:400-800 is optimal. Flow-Cyt testing requires 1μg of the sample per test, and it is important to note that paraffin sections will need antigen retrieval. These recommendations are based on the original text information and are meant to serve as a helpful guide for laboratory protocols. Please follow all established procedures and guidelines carefully to ensure accurate and reliable results.

Immunogen

A synthetic peptide originating from human VEGFR2:101-200/1356 has been conjugated to KLH in our lab. We have used this synthetic peptide to generate a highly similar content by rearranging the above content. The generated content is based on the original text information and provides detailed information about the peptide. This synthetic peptide is highly valuable for research purposes, as it can be used to study the mechanisms of VEGFR2 signaling. We have ensured that the peptide is of the highest quality, and our lab has used the latest techniques to synthesize and purify it. Scientists and researchers worldwide can use this peptide to further their research and understanding of VEGFR2 and its role in cancer and other diseases. We take pride in our research and peptide production facilities, and always strive to provide our customers with the best quality products.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

For a storage period of one year, it is recommended to keep the item at a temperature of -20 °C. It is important to avoid subjecting it to multiple freeze/thaw cycles during this time.

Storage buffer

A solution containing 0.01M TBS at pH7.4 has been prepared. The solution also contains 1% BSA, 0.03% Proclin300, and 50% Glycerol.

Research area

The field of cancer research encompasses an array of fascinating topics, including tumor cell biology, immunology, and neurobiology. An understanding of signal transduction and the role of growth factors and hormones in cancer development is crucial in this field. Key players in this process include kinases and phosphatases, as well as cell membrane receptors that help determine cell fate. The identification of cell type markers and the study of endothelial cells are both important areas of investigation to better understand cancer physiology and treatments.

Target

Background

VEGF is a vital growth factor for endothelial cells and plays a significant role in the development of blood vessels. The VEGF receptor 2, also known as kinase insert domain receptor (KDR), is a type III receptor tyrosine kinase that helps to mediate endothelial proliferation, migration, survival, tubular morphogenesis and sprouting. The functioning of this receptor is regulated by multiple factors such as integrin alphaVbeta3, T-cell protein tyrosine phosphatase, Rab GTPase and P2Y purine nucleotide receptor. Mutations in the KDR gene are associated with infantile capillary hemangiomas. This information is provided by RefSeq as of May 2009.

Celluar localization

Nucleus cytoplasm cell membrane secreted protein

UniPort

P35968