Phospho-PAK3(Ser154) Rabbit Polyclonal Antibody #abs130015

abs130015-50ug

1-2 Weeks

201

abs130015-100ug

1-2 Weeks

301

Description

PAK3 is a type of protein kinase that belongs to the STE20 family. It serves as a regulator for the formation and plasticity of synapses in the hippocampus. This protein kinase is believed to play a role in dendritic development and the rapid rearrangement of the cytoskeleton in dendritic spines, which is crucial for synaptic plasticity. PAK3 forms a complex with activated P21, CDC2, and RAC1 proteins, all of which are bound to GTP. Truncation and missense mutations in PAK3 have been associated with nonsyndromic mental retardation type 30 (MRX30). In humans, there are two different isoforms of PAK3 that have been identified through alternative splicing. It's important to note that this information may have been sourced from UniProtKB.

Other names

The PAK family of proteins includes Alpha PAK, Beta PAK, and Gamma PAK, as well as PAK 1, PAK 2, and PAK 3. Each member of this family is a serine/threonine protein kinase that is activated by the binding of GTP-bound Rho and Rac small GTPases. PAKs are involved in a variety of cellular processes, including cytoskeletal organization, cell adhesion, proliferation, and apoptosis.
Other members of the PAK family include MRX30, MRX47, and Oligophrenin 3 (OPHN3). PAK 3beta, also known as hPAK3, is a member of the PAK3 subfamily and is involved in regulating the process of synaptic plasticity. P21 (CDKN1A) activated kinase 2 and P21 (CDKN1A) activated kinase 3 are two closely related isoforms of PAK. P65 PAK, also known as PAK2, is involved in regulating the NF-kappaB signaling pathway.
Overall, the PAK family of proteins plays an important role in regulating various cellular processes, making them an important area of study in the field of cell biology. Understanding the function of each member of this family can provide valuable insights into the development and progression of diseases such as cancer and neurodegenerative disorders.

Source

Rabbit

Specificity

The presence of phosphorylated PAK3 at Serine 154 can be detected using the Phospho-PAK3(Ser154) Antibody. This antibody specifically recognizes endogenous levels of PAK3 only when it is phosphorylated at Serine 154.

Species Reactivity

Human;Mouse;Rat

Application

For Western Blot, the recommended dilution range is between 1:500 and 1:2000, while for immunohistochemistry, it is 1:50-1:200. If performing ELISA with a peptide substrate, the optimal dilution range is between 1:20000 and 1:40000. These are the suggested dilutions for optimal results based on the original content provided.

Immunogen

A highly similar content can be generated by rearranging the above information as follows:
A peptide synthesized from human PAK3 has been derived that is situated around the phosphorylation site of Sersine 154. This synthesized peptide is expected to possess a highly similar content to the original text information, as it has been specifically created to mimic the structure and properties of natural PAK3. It is anticipated that this synthesized peptide will prove to be a valuable tool in further research and investigation into the function and mechanisms of PAK3, particularly in regards to its phosphorylation and signaling processes.

Properties

Concentration

1mg/ml

Purification

By performing sequential chromatography on phospho- and non-phospho-peptide affinity columns, the purified rabbit serum yielded the desired antibody through affinity purification.

Clonality

Polyclonal Antibody

Stability & Storage

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

Tissue specificity

Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.

Posttranslational modification

Autophosphorylated when activated by CDC42/p21.Neddylated.

Celluar localization

Cytosol;Endosome;Plasma Membrane;

UniPort

O75914