Phospho-FER(Tyr402) Rabbit Polyclonal Antibody #abs130014

abs130014-50ug

1-2 Weeks

201

abs130014-100ug

1-2 Weeks

301

Description

Fer protein, a nontransmembrane receptor tyrosine kinase, belongs to the FPS/FES family. Its main role is to control cell-cell adhesion and facilitate signal transmission between the cell surface and the cytoskeleton, primarily through growth factor receptors.

Other names

FER, also known as Fer (fps/fes related) tyrosine kinase or Feline encephalitis virus related kinase, is a tyrosine kinase protein encoded by the Fer gene. It is also sometimes referred to as Phosphoprotein NCP94 or Proto-oncogene c-Fer.
This protein is involved in various signaling pathways and cellular processes, including cell proliferation, differentiation, and migration. It has been implicated in the development of several types of cancer, including lung cancer and leukemia.
FER is a member of the Fes/Fps family of non-receptor tyrosine kinases, which share a conserved catalytic domain. It has multiple isoforms, including p94 FER, which is the most abundant isoform in most tissues.
Overall, FER is an important protein with diverse functions, and further research into its role in cancer and other diseases may lead to new therapeutic targets.

Source

Rabbit

Specificity

The detection capability of Phospho-FER(Tyr402) Antibody is specifically directed towards endogenous levels of FER that exhibit phosphorylation at Tyrosine 402. To maintain consistency with the original text, the generated content needs to be based on the given information.

Species Reactivity

Human;Mouse

Application

The recommended dilutions for using this antibody in various applications are as follows: Western Blotting at a dilution range of 1:500-1:2000, Immunohistochemistry at a dilution range of 1:50-1:200, Immunofluorescence/Immunocytochemistry at a dilution range of 1:100-1:500, and ELISA(peptide) at a dilution range of 1:20000-1:40000. It is important to note these dilution ranges to ensure optimal results when using this antibody for research purposes.

Immunogen

A peptide created by synthesizing human FER, which is based around the phosphorylation site of Tyrosine 402, has been generated. The content of the peptide is highly similar to the original text information and has been rearranged to ensure accuracy.

Properties

Concentration

1mg/ml

Purification

The process of obtaining the antibody involves purifying rabbit serum, followed by affinity purification using sequential chromatography on columns specific for phospho- and non-phospho-peptides. This method ensures the purification of the desired antibodies from the serum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of the product, it is recommended to keep it stored at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing as it can adversely affect its quality.

Storage buffer

This product is Rabbit IgG dissolved in phosphate-buffered saline with a pH of 7.4, 150 millimolar sodium chloride, 0.02% sodium azide, and 50% glycerol. It is advised to store this at a temperature of minus 20 degrees Celsius, and it should remain stable for up to twelve months from the time you receive it.

Target

Background

Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus.

Tissue specificity

Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.

Posttranslational modification

Autophosphorylated.Polyubiquitinated; this leads to proteasomal degradation.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;Plasma Membrane;

UniPort

P16591