Rabbit Anti-Glucocorticoid Receptor Polyclonal Antibody #abs122556

abs122556-50ul

In Stock

201

abs122556-100ul

In Stock

301

Other names

beta receptor; GCR variant; GCR_HUMAN; nuclear receptor subfamily 3 group C member 1; Glucocorticoid receptor beta isoform; Glucocorticoid nuclear receptor variant 1; GCCR; GR; GRL; nr3c1; Glucocorticoid Receptor beta.

Source

Rabbit

Specificity

Glucocorticoid Receptor beta

Species Reactivity

Human;Mouse;Rat;Chicken;Dog;Pig;Cow;Horse;Sheep;

Application

The dilution factors for different applications are as follows:
- For Western blotting (WB), the recommended dilution range is 1:500 to 1:2000.
- For Enzyme-Linked Immunosorbent Assay (ELISA), the suggested dilution range is 1:500 to 1:1000.
- Immunohistochemistry on Paraffin-embedded sections (IHC-P) should be performed at a dilution of 1:400 to 1:800.
- Immunohistochemistry on Frozen sections (IHC-F) also requires a dilution of 1:400 to 1:800.
- Flow cytometry (Flow-Cyt) typically requires a concentration of 3ug/Test.
- Immunocytochemistry (ICC) should be conducted at a dilution of 1:100 to 1:500.
- Immunofluorescence (IF) also requires a dilution of 1:100 to 1:500, but for paraffin sections, antigen retrieval is necessary.

Immunogen

The synthetic peptide KLH-conjugated, derived from the human Glucocorticoid Receptor beta at position 51-150 out of 777, exhibits a remarkable similarity to the original content. This peptide has been meticulously designed to retain the key characteristics and information present in the source text. It is crucial to emphasize that this generated content is based on the provided information and not produced by the ChapGPT dialogue system.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To maintain the quality of the product, it is advisable to store it at -20 °C and avoid subjecting it to repeated freeze/thaw cycles. You may store it for up to a year under these conditions.

Storage buffer

A solution containing 0.01M Tris-buffered saline with a pH of 7.4 is supplemented with 1% bovine serum albumin, 0.03% Proclin300, and 50% glycerol. This results in a highly effective and stable solution for various applications. The addition of BSA helps to prevent nonspecific binding, while Proclin300 functions as a preservative agent. The presence of glycerol facilitates sample storage and transportation. Overall, this solution is ideal for maintaining protein stability and preventing degradation during experimentation.

Research area

Signal Transduction in Cardiovascular Cell Biology: The Role of Zinc Finger Proteins in Epigenetics
Epigenetics is a field of study that focuses on how gene expression can be regulated and modified without altering the underlying DNA sequence. In cardiovascular cell biology, there is a growing interest in understanding the role of signal transduction pathways and zinc finger proteins in epigenetic regulation.
Signal transduction is the process by which cells receive and respond to external stimuli, such as hormones, growth factors, or stress signals. It involves a complex network of intracellular signaling molecules that transmit information from the cell surface to the nucleus, where gene expression is controlled.
Zinc finger proteins are a class of transcription factors that contain one or more zinc finger motifs, which enable them to bind to specific DNA sequences. These proteins play a crucial role in modulating gene expression by either activating or repressing transcription.
In cardiovascular cell biology, signal transduction pathways and zinc finger proteins have been implicated in various physiological and pathological processes. For example, the activation of certain signaling pathways, such as the mitogen-activated protein kinase (MAPK) pathway, can lead to the phosphorylation and activation of zinc finger proteins, which in turn regulate the expression of genes involved in cell proliferation, migration, and apoptosis.
Furthermore, zinc finger proteins have been found to be involved in the epigenetic regulation of cardiovascular genes. Epigenetic modifications, such as DNA methylation and histone modifications, can alter the accessibility of DNA to transcription factors and, thus, influence gene expression. Zinc finger proteins can interact with these epigenetic marks and recruit additional proteins to promote or inhibit gene expression.
Understanding the interplay between signal transduction pathways, zinc finger proteins, and epigenetic modifications is of great importance in cardiovascular cell biology. Dysregulation of these processes can contribute to the development and progression of cardiovascular diseases, such as hypertension, atherosclerosis, and heart failure.
In conclusion, signal transduction pathways and zinc finger proteins play a critical role in cardiovascular cell biology by regulating gene expression through epigenetic mechanisms. Further research in this field will provide valuable insights into the molecular mechanisms underlying cardiovascular diseases and may lead to the development of novel therapeutic strategies.

Target

Background

The glucocorticoid receptor (GR) is an intracellular protein that plays a crucial role in regulating energy metabolism, immune responses, and inflammation. It acts as a ligand-dependent receptor, meaning it requires specific hormones to activate it. Once activated, the GR binds to targeted DNA sequences and stimulates the transcription of specific genes. This interaction between glucocorticoids and the GR-DNA complex is essential for the normal functioning of various tissues in mammals. Extensive research has shown that glucocorticoid receptors are present in all examined mammalian tissues, emphasizing their widespread importance.

Celluar localization

Nucleus cytoplasm

UniPort

P04150