EIF2S1 Polyclonal Antibody #abs115171

abs115171-50ul

In Stock

228

Other names

EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A

Source

Rabbit

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Immunogen

A highly similar recombinant fusion protein can be developed by incorporating a protein sequence that corresponds to amino acids 1-260 of the human EIF2S1 (NP_004085.1). This fusion protein will retain the essential characteristics and functionalities of the original sequence while potentially offering enhanced properties.

Properties

Mouse kidney and mouse spleen are two types of mouse tissues. LO2, Jurkat, MCF-7, SW480, and SKOV3 are different cell lines.

Properties

Purification

Affinity purification

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
The pH of the buffer is 7.3 and it contains 0.02% sodium azide and 50% glycerol. Additionally, it is comprised of PBS with a final volume of solution made up to a total of 100%.

Target

Background

The initiation of protein synthesis is controlled by EIF2, an important translation initiation factor. Its main function is to facilitate the binding of the initiator tRNA to the 40S ribosomal subunits, which is the first step in protein synthesis. This binding occurs in the form of a ternary complex composed of methionyl-tRNA, EIF2, and GTP. EIF2 itself is made up of three subunits: the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate at which the ternary complex is formed is regulated by the phosphorylation status of the EIF2-alpha subunit. This important aspect was discovered by Ernst et al. in 1987 (PubMed 2948954). In summary, EIF2 plays a crucial role in the regulation of protein synthesis initiation through its interaction with the initiator tRNA and ribosomal subunits, and this process is influenced by the phosphorylation state of the EIF2-alpha subunit.

Celluar localization

Cytoplasmic granule

UniPort

P05198