Rabbit Anti-TF2H2 Polyclonal Antibody #abs130446

abs130446-50ug

1-2周

￥1100.00

abs130446-100ug

1-2周

￥1650.00

Description

On chromosome 5q13, there exists a 500 kb inverted duplication which encompasses this particular gene. Within this duplicated region, there are multiple genes and repetitive elements that increase the likelihood of rearrangements and deletions. The intricate and repetitive nature of the sequence has posed challenges in deciphering the organization of this genomic region. Specifically, this gene is located within the telomeric copy of the duplication. In some cases of spinal muscular atrophy (SMA) patients, the deletion of this gene coincides with the deletion of the neighboring SMN1 gene; however, it remains uncertain whether the absence of this gene contributes to the SMA phenotype. The encoded protein of this gene is the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH, which plays a role in basal transcription and nucleotide excision repair. Although transcript variants of this gene have been identified, their complete structure is yet to be determined. Interestingly, a second copy of this gene has been reported in the literature, located within the centromeric copy of the duplication. Notably, there are contradicting reports stating a difference of either two or four base pairs between the two copies. It is important to note that there is currently no available sequence data for the centromeric copy of the gene.

Other names

The 44 kDa subunit of basic transcription factor 2, also known as BTF2, BTF2 p44, BTF2-p44, BTF2P44, or T BTF2P44, plays a crucial role in the regulation of gene expression. It is a component of the General Transcription Factor IIH (TFIIH), which is responsible for initiating transcription by RNA polymerase II. TFIIH is composed of multiple subunits, including the General Transcription Factor IIH Polypeptide 2 (Gtf2h2) or General Transcription Factor IIH Subunit 2 (TFIIH subunit 2) with a molecular weight of 44 kDa. Another name for this subunit is p44. TFIIH is a complex multi-protein assembly involved in various cellular processes, including transcription, DNA repair, and cell cycle control. Its involvement in these processes highlights the significance of the 44 kDa subunit in ensuring proper gene regulation and cellular homeostasis.

Source

Rabbit

Specificity

TF2H2 Antibody detects endogenous levels of TF2H2

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Immunogen

A synthesized peptide derived from human TF2H2

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this may compromise its quality and efficacy. Therefore, it is advisable to take necessary precautions and handle the product with care during storage and handling. By following these guidelines, you can help maintain the integrity of the product and ensure its effectiveness over time.

Storage buffer

This is Rabbit IgG in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store it at -20 °C and it is stable for 12 months from the date of receipt. To summarize, this is a long-term stable solution of Rabbit IgG that is convenient to store and can be used for a variety of applications.

Target

Background

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.

Tissue specificity

Widely expressed, with higher expression in skeletal muscle.

Celluar localization

Nucleus;

UniPort

Q13888