Rabbit Anti-MLK4 Polyclonal Antibody #abs130447

abs130447-50ug

1-2 Weeks

201

abs130447-100ug

1-2 Weeks

301

Description

MLK4 is a member of the protein kinase superfamily and belongs to the STE Ser/Thr protein kinase family, specifically the MAP kinase kinase kinase subfamily. This protein activates the JUN N-terminal pathway and is known to form homodimers. There are three human isoforms of MLK4 produced through alternative splicing. It's worth noting that this description may contain information from UniProtKB, a protein knowledgebase.

Other names

MLK4, also known as mixed lineage kinase 4, is a mitogen-activated protein kinase kinase kinase that plays a crucial role in signal transduction pathways. Its primary function is to activate the downstream effectors of the mitogen-activated protein kinase pathway, which ultimately leads to cellular proliferation, differentiation, and survival. KIAA1804 is the gene that codes for MLK4, and M3KL4_HUMAN is its protein ID. The RP5 862P8.2 variant of the MLK4 gene has also been identified.
In summary, MLK4 is a vital component of the MAPK signaling pathway and is involved in numerous cellular processes. Its identification via the KIAA1804 gene and the RP5 862P8.2 variant can aid in the understanding of disease pathogenesis and potential therapeutic targets.

Source

Rabbit

Specificity

The protein MAP3KL4 can be detected using the MAP3KL4 antibody at levels that occur naturally within the body. It is important to note that this antibody is effective in identifying endogenous MAP3KL4 protein.

Species Reactivity

Human

Application

The recommended dilutions for Western blotting range from 1:500 to 1:2000, for immunofluorescence or immunocytochemistry they range from 1:100 to 1:500, and for ELISA using a peptide they range from 1:20000 to 1:40000. It is important to carefully consider these dilutions to ensure optimal results in your experiments.

Immunogen

A synthesized peptide derived from human MAP3KL4.

Properties

Concentration

1mg/ml

Purification

To obtain a purified antiserum, we utilized SulfoLink™ Coupling Resin for peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To prevent damage, store for one year at -20 °C and refrain from subjecting it to multiple freeze/thaw cycles. Make sure to handle the original text information while generating a substantially different content to avoid following the conversation method used by ChapGPT.

Storage buffer

This solution contains Rabbit IgG in phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain its stability, it should be stored at -20 °C. The shelf life of this product is 12 months from the date of receipt. Feel free to let me know if you would like more information or assistance with anything else.

Target

Background

This particular regulator has a negative impact on the signaling of TLR4. Its actions do not involve the activation of the JNK1/MAPK8 pathway, nor the p38/MAPK14 or ERK2/MAPK1 pathways.

Posttranslational modification

Enzyme activation is facilitated by autophosphorylation occurring within the activation loop at serine and threonine residues.

UniPort

Q5TCX8