Rabbit Anti-Dysferlin Polyclonal Antibody #abs130087

abs130087-50ug

1-2 Weeks

201

abs130087-100ug

1-2 Weeks

301

Description

DYSF, a calcium ion sensor, is an essential component in the process of Ca(2+)-triggered fusion between synaptic vesicles and the plasma membrane. It plays a crucial role in the repair mechanism of both skeletal muscle and cardiomyocytes by facilitating the rapid resealing of membrane disruptions caused by mechanical stress. This protein is a member of the ferlin family and is known to interact with CACNA1S. Additionally, it demonstrates an interaction with ANXA1, which is dependent on the presence of Ca(2+) and the state of injury.

Other names

Dysferlin is a protein that is also known as Dystrophy-associated fer-1-like protein, Fer-1-like protein 1 or Fer1 like protein 1. The Dysferlin gene is located on chromosome 2 in humans and is responsible for encoding Dysferlin. This protein is primarily found in muscle fibers and is involved in the repair and maintenance of these cells.
Dysferlin mutations are associated with several muscular dystrophy conditions, including Limb girdle muscular dystrophy 2B (LGMD 2B) and Miyoshi myopathy (MM). These disorders are inherited in an autosomal recessive manner and are characterized by progressive muscle weakness and atrophy.
Research into Dysferlin and its associated conditions is ongoing, with a focus on developing therapies that can help to slow or even reverse the muscle wasting that is characteristic of these disorders. Improved understanding of the function of Dysferlin may also pave the way for the development of targeted interventions that can address the underlying causes of these conditions.

Source

Rabbit

Specificity

The ability of the Dysferlin antibody lies in its detection of the total Dysferlin levels present within the body, thus allowing for the assessment of endogenous Dysferlin levels.

Species Reactivity

Human;Mouse

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

Western blot (WB) is typically performed using antibody dilutions ranging from 1:500 to 1:3000. For immunofluorescence (IF) or immunocytochemistry (ICC), the recommended antibody dilutions are usually 1:100 to 1:500. When performing ELISA with peptide antigens, it is common to use antibody dilutions of 1:20000 to 1:40000.

Immunogen

Dysferlin, a protein found in humans, can be used to create a synthesized peptide. This peptide is derived from human Dysferlin and retains a high level of similarity. By rearranging the given information, the resulting content is based on the original text and provides an alternative representation.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was used to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product, it is recommended to store it at -20 °C for one year. It is important to avoid subjecting it to repeated cycles of freezing and thawing.

Storage buffer

The storage conditions for Rabbit IgG include storing it at -20 °C in phosphate buffered saline with a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that the stability of the IgG is guaranteed for 12 months from the date of receipt. Please keep in mind that this generated content may be different from the original text information.

Target

Background

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).

Tissue specificity

Expressed in skeletal muscle, myoblast, myotube and in the syncytiotrophoblast (STB) of the placenta (at protein level). Ubiquitous. Highly expressed in skeletal muscle. Also found in heart, brain, spleen, intestine, placenta and at lower levels in liver, lung, kidney and pancreas.

Celluar localization

Cytoskeleton;Endosome;Extracellular region or secreted;Plasma Membrane;

UniPort

O75923