Rabbit Anti-HDAC1 Polyclonal Antibody #abs130115

abs130115-50ug

1-2 Weeks

201

abs130115-100ug

1-2 Weeks

301

Description

A crucial member of the histone deacetylase family, subfamily 1, is HDAC1, which regulates transcription. It accomplishes this by removing acetyl groups from lysine residues situated at the N-terminal of the core histones H2A, H2B, H3, and H4. As a result, HDAC1 is responsible for controlling the cell cycle progression, developmental events, and transcriptional regulation.

Other names

The protein DKFZp686H12203, also known as HDAC 1 or Histone deacetylase 1, plays a key role in regulating gene expression through its ability to deacetylate histones. It is also referred to as Reduced potassium dependency yeast homolog like 1 and RPD3L1. This protein is essential for many cellular processes such as chromatin remodeling, cell cycle progression and differentiation. Its dysfunction has been linked to various diseases like cancer, Alzheimer's, and heart disease. GON 10 is another name for this protein, which helps in identifying it in research studies. In summary, HDAC1 or DKFZp686H12203 is a vital protein that maintains the proper functioning of cells and is implicated in several human diseases.

Source

Rabbit

Specificity

The HDAC1 antibody is capable of detecting total HDAC1 levels in the body's own cells, without any need for external interference or manipulation. To ensure accuracy of detection, the antibody has been tested on various samples and has consistently produced reliable results. It is an essential tool for researchers and scientists studying the functions of HDAC1 in different biological processes. By detecting endogenous levels of HDAC1, the antibody provides valuable insights into the regulation and activity of this important enzyme.

Species Reactivity

Human;Mouse;Rat

Application

For Western blotting, a dilution of 1:500 to 1:3000 is recommended, while immunohistochemistry requires a dilution of 1:50 to 1:200. For imaging techniques like immunofluorescence and immunocytochemistry, a dilution of 1:100 to 1:500 is recommended. For ELISA using peptide substrates, a dilution range of 1:20000-1:40000 is optimal. These dilutions have been tested and validated for accurate and reliable results in various applications. It's important to carefully optimize your dilution for each specific experiment to ensure accurate and reproducible results.

Immunogen

A synthesized peptide derived from human HDAC1.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain its quality, the product should be stored at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

This product is Rabbit IgG that comes in a buffered saline solution with a pH of 7.4. It also contains 150mM of NaCl, 0.02% sodium azide, and 50% glycerol to maintain its stability. To keep it fresh, it's advised to store it at a temperature of -20°C, where it can stay viable for up to 12 months from the date of receipt.

Target

Background

Histone deacetylases are responsible for the deacetylation of lysine residues on the N-terminal portion of core histones (H2A, H2B, H3, and H4). This process contributes to epigenetic repression and plays a crucial role in regulating transcription, cell cycle progression, and developmental events. Histone deacetylases function through the formation of large multiprotein complexes. They deacetylate SP proteins, specifically SP1 and SP3, to regulate their function. HDAC1 is a component of the BRG1-RB1-HDAC1 complex, which negatively regulates CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex, and CREBBP is recruited to facilitate transcriptional activation. Additionally, HDAC1 deacetylates TSHZ3 and modulates its transcriptional repressor activity. It also deacetylates 'Lys-310' on RELA, inhibiting the transcriptional activity of NF-kappa-B. HDAC1 has a role in abrogating KAT5-mediated relief of NR1D2 transcription repression activity by deacetylating NR1D2. It is part of the RCOR/GFI/KDM1A/HDAC complex, which suppresses the expression of several genes involved in multilineage blood cell development by recruiting histone deacetylases (HDACs). HDAC1 is involved in the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.

Tissue specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Posttranslational modification

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Phosphorylated by CDK5.Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by KCTD11, leading to proteasomal degradation.

Celluar localization

Cytosol;Nucleus;

UniPort

Q13547