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Rabbit Anti-HDAC10 Polyclonal Antibody #abs130116

Dear customers, Please note that the price provided above is only for your reference. For more detailed pricing information, we kindly request you to contact our seller, Vecent. Thank you for your understanding. Data Examples Western blot analysis on COS7 cell lysate using HDAC10 Antibody This...

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Description

Catalog-specification	Delivery time	USD price
abs130116-50ug	1-2 Weeks	201
abs130116-100ug	1-2 Weeks	301

Dear customers,
Please note that the price provided above is only for your reference. For more detailed pricing information, we kindly request you to contact our seller, Vecent.
Thank you for your understanding.

Overview
Description	Histone deacetylase 10 (HDAC10) is an enzyme responsible for removing acetyl groups from lysine residues at the N-terminal region of core histones, specifically H2A, H2B, H3, and H4. This deacetylation process plays a crucial role in epigenetic repression, transcriptional regulation, cell cycle progression, and developmental events. HDAC10 belongs to the histone deacetylase family, specifically the type 2 subfamily. It operates by forming large multiprotein complexes and interacts with other proteins such as HDAC2, HDAC3, and NCOR2. In humans, there are four isoforms of HDAC10 that are produced through alternative splicing mechanisms.
Other names	Histone deacetylase 10, also known as HDAC 10 or HDA10_HUMAN, is encoded by the gene DKFZP761B039. It is also referred to as MGC149722 or OTTHUMP00000028555. HDAC 10 plays a crucial role in regulating gene expression by removing acetyl groups from histone proteins. This process can lead to chromatin condensation and transcriptional repression. HDAC 10 is a promising target for therapeutic intervention in various diseases, including cancer and neurological disorders. Extensive research efforts are being directed towards understanding its molecular mechanisms and developing specific inhibitors. The significance of HDAC 10 in chromatin remodeling and epigenetic regulation highlights its potential as a key player in cellular processes and disease pathogenesis.
Source	Rabbit
Specificity	The detection of endogenous levels of total HDAC10 can be achieved using the HDAC10 antibody. This antibody specifically targets and recognizes HDAC10, allowing for the accurate measurement of its presence in cells or tissues. By detecting the total HDAC10 protein, researchers can gain insights into its role and function in biological processes. This antibody-based method provides a reliable and efficient means to study HDAC10 in different experimental settings.
Species Reactivity	Human;Mouse;Rat;Monkey
Application	The recommended dilutions for Western blot (WB) range from 1:500 to 1:3000. For immunohistochemistry (IHC), the suggested dilutions are 1:50 to 1:200. In the case of immunofluorescence/immunocytochemistry (IF/ICC), the optimal dilutions are 1:100 to 1:500. Lastly, for ELISA using peptide antigens, it is advised to dilute the samples at 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human HDAC10.
Properties
Concentration	1mg/ml
Purification	To obtain the purified antiserum, SulfoLink™ Coupling Resin was used for peptide affinity chromatography. The process involved separating the target peptides from other components. This ensured that the final product was highly purified and of high quality.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of the product, it is recommended to store it at a low temperature of -20°C for a period of one year. It is important to avoid any repeated cycles of freezing or thawing, as this may compromise the quality of the item. We advise following these storage guidelines to maintain the integrity of the product.
Storage buffer	This product contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, as well as 150 mM of NaCl, 0.02% sodium azide, and 50% glycerol. It is advised to store this product at a temperature of -20°C, and it remains stable for 12 months from the date of its receipt.
Target
Background	The N-terminal part of the core histones (H2A, H2B, H3 and H4) is subject to deacetylation by the enzyme known as histone deacetylase. This process gives rise to a tag for epigenetic repression and plays a crucial role in the regulation of transcription, cell cycle progression, as well as developmental events. Histone deacetylases operate through the establishment of elaborate multiprotein complexes that carry out their function.
Tissue specificity	Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Celluar localization	Nucleus;
UniPort	Q969S8