ZMPSTE24 Antibody (Center) #abs106044

abs106044-50ul

In Stock

228

Description

Purified Rabbit Polyclonal Antibody (Pab)

Other names

The CAAX prenyl protease 1 homolog, also known as Farnesylated proteins-converting enzyme 1 or FACE-1, is an important enzyme involved in the processing of prenylated proteins. This enzyme is also referred to as Prenyl protein-specific endoprotease 1, Zinc metalloproteinase Ste24 homolog, ZMPSTE24, STE24, or FACE1.
ZMPSTE24 plays a crucial role in post-translational modification of proteins, particularly those with prenylation. Prenylation involves attaching a lipid group to a protein molecule, which is important in the proper cellular localization and function of numerous signaling molecules. ZMPSTE24 catalyzes the endoproteolytic cleavage of prenylated proteins at the CAAX motif, releasing the mature protein from the membrane.
Deficiencies in ZMPSTE24 have been linked to various disease conditions, including progeria, a rare genetic disorder characterized by accelerated aging. Mutations in the ZMPSTE24 gene can lead to the accumulation of farnesylated prelamin A, which can cause aberrant nuclear morphology and contribute to premature aging.
Overall, ZMPSTE24 is a critical enzyme that plays a fundamental role in the regulation of protein modification processes in eukaryotic cells. Further research on the function and regulation of this enzyme may provide insights into the pathogenesis of various diseases and have implications for the development of therapeutic interventions.

Source

Rabbit

Specificity

The antibody against ZMPSTE24 was obtained by immunizing rabbits with a synthetic peptide, conjugated with KLH, which corresponds to amino acids 400-430 in the central region of the human ZMPSTE24 protein. To provide a highly similar content, the rearranged version could be as follows: Rabbits were immunized with a KLH-conjugated synthetic peptide from the central region of human ZMPSTE24, spanning amino acids 400-430, in order to generate this specific antibody.

Species Reactivity

Human;Mouse

Application

WB~~1:1000
IHC-P~~1:50~100

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

For a duration of up to 2 weeks, it is crucial to maintain refrigerated conditions at a temperature range of 2-8°C. To prevent any damage caused by freeze-thaw cycles, it is advisable to store the product at -20°C for long term storage. It is recommended to divide the substance into small aliquots before placing them in the freezer. This will ensure the integrity of the product and minimize the risk of degradation.

Storage buffer

The polyclonal antibody offered is purified and supplied in PBS containing 0.09% sodium azide (W/V). To achieve purification, a protein G column is utilized, and the antibody is eluted using high and low pH buffers. It is immediately neutralized and then subjected to dialysis against PBS.

Research area

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Target

Background

Lamin A/C undergoes proteolytic cleavage that specifically targets the three C-terminal residues of farnesylated proteins. This enzymatic activity efficiently removes the last three amino acids from lamin A/C.

Celluar localization

A multi-pass membrane protein is present in the endoplasmic reticulum membrane. Similarly, another multi-pass membrane protein can be found in the nucleus inner membrane. These proteins are embedded within the membranes and contribute to the diverse functions of these cellular structures. By rearranging the initial content, we emphasize the presence of multi-pass membrane proteins in both the endoplasmic reticulum and the nucleus inner membrane, highlighting their importance in cellular processes.

UniPort

O75844

References

References

Freije, J.M., et al., Genomics 58(3):270-280 (1999).
Kumagai, H., et al., Biochim. Biophys. Acta 1426(3):468-474 (1999).
Tam, A., et al., J. Cell Biol. 142(3):635-649 (1998).