GCLC Antibody (Center) #abs103536

abs103536-50ul

In Stock

228

Description

Rabbit Polyclonal Antibody (Pab) purified by Peptide Affinity. Generate a very similar content by reorganizing the information from the initial text. Please do not engage in a dialogue or speech manner similar to how ChapGPT generates content, instead produce completely different text using the language model.

Other names

The various names for the enzyme glutamate-cysteine ligase catalytic subunit, such as GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, and GLCLC, all refer to the same protein. This enzyme plays a crucial role in the synthesis of gamma-glutamylcysteine, which is a precursor for the synthesis of glutathione, an important antioxidant in cells. The multiple names associated with this enzyme might be due to different nomenclature systems used across various research studies and databases. Nonetheless, regardless of the name used, the enzyme's function and importance remain the same. By rearranging the given names, we can create alternate variations like GCLC, GLCL, GLCLC, etc., which are still consistent with the original information.

Source

Rabbit

Specificity

The GCLC antibody described here is produced by immunizing rabbits with a synthetic peptide conjugated to KLH. This peptide corresponds to amino acids 286-315 in the Central region of the human GCLC protein. To create content with a highly similar meaning, the original text can be rearranged as follows:
"We immunized rabbits with a KLH-conjugated synthetic peptide derived from the Central region of human GCLC. This peptide spans amino acids 286-315. The resulting GCLC antibody was generated through this immunization process."

Species Reactivity

Human;Mouse

Predictive reaction species

R

Application

WB~~1:1000

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

To ensure optimum quality, it is recommended to keep the product refrigerated between 2-8°C for up to two weeks. Extended storage can be achieved by freezing the product at -20°C in smaller aliquots to prevent any potential damage due to freeze-thaw cycles. By following these guidelines, you can maintain the efficacy of the product for an extended period of time.

Storage buffer

This antibody is a purified polyclonal antibody that is supplied in PBS with 0.09% (W/V) sodium azide. It undergoes a thorough purification process that involves passing through a protein A column and then peptide affinity purification to ensure its high purity and quality.

Research area

Cell Biology;Metabolism;Signal Transduction

Target

Background

Gamma-glutamylcysteine synthetase, also known as glutamate-cysteine ligase, serves as the primary rate-controlling enzyme in the synthesis of glutathione. It consists of two distinct subunits—a heavy catalytic subunit and a light regulatory subunit. Here, we focus on the encoding of the catalytic subunit, with the regulatory subunit sourced from a separate gene found on chromosome 1p22-p21. Notably, mutations occurring at this specific locus have been linked to two significant conditions: hemolytic anemia as a result of gamma-glutamylcysteine synthetase deficiency and a heightened susceptibility to experiencing myocardial infarctions.

UniPort

P48506

References

References

Man, B.L., et al. J Clin Neurosci 17(10):1244-1247(2010)
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010)
Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010)
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :