SQSTM1 Polyclonal Antibody #abs118097

abs118097-50ul

In Stock

228

Other names

A170;DMRV;FTDALS3;NADGP;OSIL;p60;p62;p62B;PDB3;ZIP3;A170;FTDALS3;OSIL;p60;p62;p62B;PDB3;ZIP3

Source

Rabbit

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Immunogen

SQSTM1，SQSTM11-440（NP_001135770.1）。，。

Properties

Jurkat, HeLa, BT-474, NCI-H460, Mouse kidney, Mouse liver. It is important to note that the generated content should be rearranged based on the original text information.

Properties

Purification

Affinity purification

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
To create a buffer solution, prepare PBS and add 0.02% sodium azide and 50% glycerol. Adjust the pH to 7.3 for optimal performance. Remember to handle sodium azide with care as it is toxic. The addition of glycerol can increase the stability of the solution and also assist in preventing freezing at low temperatures. The resulting buffer will be suitable for various applications, such as protein purification and enzyme assays.

Target

Background

This protein, which is encoded by a single gene, possesses multiple functions that involve binding to ubiquitin and controlling the activation of the nuclear factor kappa-B (NF-kB) signaling pathway. Acting as an adaptor protein alongside TNF receptor-associated factor 6, it plays an important role in mediating NF-kB activation in response to upstream signals, and hence functions as a scaffold. Numerous alternatively spliced transcript variants of this same gene have been identified, encoding either the same or different isoforms. However, mutations in this gene can give rise to both sporadic and familial Paget disease of bone.

Celluar localization

The cell is composed of various organelles like cytoplasm, nucleus, and endoplasmic reticulum that perform specific functions. In addition, there are also other important structures like lysosomes, late endosomes, cytoplasmic vesicles, P-bodies, and autophagosomes. These structures play crucial roles in cellular transport, storage, and degradation of substances.
The cytoplasm is a gel-like substance that fills the cell and contains various organelles including the endoplasmic reticulum and the cytoplasmic vesicles. The endoplasmic reticulum is responsible for synthesizing proteins, lipids, and other important substances, while the cytoplasmic vesicles are involved in the transport of various molecules within the cell.
The nucleus is the control center of the cell, which contains genetic material in the form of DNA. It is responsible for various cellular processes like cell division, growth, and development. The P-body is a cytoplasmic structure that is involved in mRNA degradation. Late endosomes are organelles that are involved in the maturation and transport of lysosomes. Lysosomes are responsible for breaking down cellular waste and foreign particles.
Finally, autophagosomes are organelles that are involved in the process of autophagy where cells break down their own cellular components for recycling. Overall, the various organelles and structures within the cell work together to ensure that the cell functions properly and efficiently.

UniPort

Q13501