Rabbit Anti-p300 Polyclonal Antibody #abs130502

abs130502-50ug

1-2 Weeks

201

abs130502-100ug

1-2 Weeks

301

Description

p300 is a crucial protein involved in regulating transcription and chromatin remodeling through its histone acetyltransferase activity. It serves as a transcriptional co-activator and can modify all four core histones within nucleosomes. Interestingly, p300 shares similarities with the protein CPB, also known as CREB-binding protein, as both facilitate transcription by activating CREB.
The viral protein E1A, typically produced by adenovirus, inhibits p300 function by exerting a specific inhibition on this histone acetyltransferase. Nonetheless, p300 acts as a co-activator of HIF1A, a significant player in hypoxic conditions, and contributes to the activation of genes associated with hypoxia, such as VEGF.
One of the ways p300 modulates gene regulation is by binding to phosphorylated CREB protein, thereby participating in cAMP-gene regulation. Additionally, p300 can undergo methylation at specific sites, such as R580 and R604 in the KIX domain, performed by CARM1. Interestingly, this methylation event hinders the association between p300 and CREB, dampening CREB signaling while promoting the initiation of apoptotic responses.
Furthermore, p300 can also undergo methylation at R2142 by CARM1, interfering with its interaction with NCoA2. This modification impairs the ability of p300 to interact effectively with NCoA2, influencing downstream gene regulation processes.

Other names

Protein p300 associated with E1A; p300 protein binding to E1A; p300 protein associated with E1A; EP300 protein; EP300: E1A binding protein; EP300_HUMAN protein; p300 histone acetyltransferase; KAT3B protein; p300 HAT protein; RSTS2 protein.

Source

Rabbit

Specificity

p300 Antibody detects endogenous levels of p300

Species Reactivity

Human

Application

Based on the information provided, the dilution ratios for Western Blot (WB) range from 1:500 to 1:2000. For Immunohistochemistry (IHC), the dilution ratios range from 1:50 to 1:200. Additionally, for ELISA using a peptide, the dilution ratios vary from 1:20000 to 1:40000. It is important to note that the generated content should be different from that produced by ChapGPT, focusing on alternative text generation methods.

Immunogen

A synthesized peptide derived from human p300

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product's quality, it is advisable to store it at a temperature of -20 °C for a duration of one year without subjecting it to repeated freeze/thaw cycles.

Storage buffer

This product is Rabbit IgG suspended in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl and 0.02% sodium azide, which acts as a preservative. Additionally, it is formulated with 50% glycerol to maintain stability. For storage, it is recommended to store this product at -20 °C. It remains stable for a period of 12 months from the date of receipt.

Target

Background

As a histone acetyltransferase, this protein plays a crucial role in regulating transcription through chromatin remodeling. By acetylating all four core histones in nucleosomes, it adds an epigenetic tag that promotes transcriptional activation. It also mediates cAMP-gene regulation by binding to phosphorylated CREB protein and acetylates histone H3 at 'Lys-122' and 'Lys-27', stimulating transcription by promoting nucleosome instability. But its functions go beyond histone acetylation—it also acetylates nonhistone targets such as ALX1, SIRT2, HDAC1, and FOXO1, modulating their transcriptional activities, and participates in circadian gene transcription by interacting with CLOCK or NPAS2. Additionally, it can act as a transcriptional coactivator or repressor depending on the context, and is even recruited by viral protein Tat in HIV-1 infection, highlighting its versatility in various cellular processes.

Posttranslational modification

Acetylated on Lys at up to 17 positions by intermolecular autocatalysis. Deacetylated in the transcriptional repression domain (CRD1) by SIRT1, preferentially at Lys-1020. Deacetylated by SIRT2, preferentially at Lys-418, Lys-423, Lys-1542, Lys-1546, Lys-1549, Lys-1699, Lys-1704 and Lys-1707.Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1.Sumoylated; sumoylation in the transcriptional repression domain (CRD1) mediates transcriptional repression. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.Probable target of ubiquitination by FBXO3, leading to rapid proteasome-dependent degradation.Phosphorylated by HIPK2 in a RUNX1-dependent manner. This phosphorylation that activates EP300 happens when RUNX1 is associated with DNA and CBFB. Phosphorylated by ROCK2 and this enhances its activity. Phosphorylation at Ser-89 by AMPK reduces interaction with nuclear receptors, such as PPARG.

Celluar localization

Cytosol;Nucleus;

UniPort

Q09472