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Rabbit Anti-RAD18 Polyclonal Antibody #abs130499

Description

Catalog-specification	Delivery time	USD price
abs130499-50ug	1-2 Weeks	201
abs130499-100ug	1-2 Weeks	301

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Overview
Description	RAD18 is a critical protein involved in the maintenance of chromosome structure, specifically in repairing UV-induced damage to DNA after replication. It exhibits an important function in binding to single-stranded DNA (ssDNA). RAD18's interaction with Rad6, mediated by a conserved ring-finger motif, is noteworthy. Rad6, an enzyme responsible for the conjugation of ubiquitin, plays a crucial role in the post-replication repair of DNA that has suffered damage. In summary, RAD18, with its ssDNA binding activity and association with Rad6, contributes significantly to the repair and integrity of UV-damaged DNA.
Other names	The gene 2810024C04Rik encodes for a DNA repair protein known as rad18. This protein functions as an E3 ubiquitin-protein ligase, specifically belonging to the RING finger protein family. It is involved in postreplication repair, where it plays a crucial role in the recognition and repair of DNA damage. In humans, rad18 is also referred to as hHR 18, hHR18, hRAD 18, hRAD18, MGC156682, or RAD18 homolog. In yeast, the rad18 homolog is essential for maintaining genomic stability and is therefore named RAD18 S. cerevisiae homolog. Another variant of rad18, known as RAD18 transcript variant, has also been identified. Additionally, this protein is referred to as RAD18 sc in some instances. RAD18 is essential for protecting cells from radiation-induced damage. It is involved in the maintenance of chromosome structure, as well as DNA repair processes. Its role as a postreplication repair protein allows it to prevent the accumulation of mutations and maintain the integrity of the genome. Overall, RAD18 plays a critical role in DNA repair mechanisms and is vital for maintaining genomic stability. Its functions as an E3 ubiquitin-protein ligase and its involvement in postreplication repair make it a key player in safeguarding the integrity of DNA.
Source	Rabbit
Specificity	RAD18 Antibody detects endogenous levels of RAD18.
Species Reactivity	Human;Rat
Predictive reaction species	Pig;Sheep;Dog
Application	The recommended dilution for Western blot is 1:500-1:2000, while the suggested dilution for immunofluorescence and immunocytochemistry is 1:100-1:500. For ELISA utilizing peptide, the dilution range suggested is 1:20000-1:40000. To ensure accurate results, it is important to follow the recommended dilution ranges for each application. By doing so, you can obtain the optimal signals for your experiments. Don't hesitate to consult the product manual or contact the technical support team if you have any questions or concerns.
Immunogen	A synthesized peptide derived from human RAD18.
Properties
Concentration	1mg/ml
Purification	SulfoLink™ Coupling Resin was employed in the purification of the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can greatly impact its shelf life and efficacy. By adhering to these guidelines, you can help ensure the longevity and effectiveness of your product.
Storage buffer	The storage condition for Rabbit IgG is in phosphate buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20°C. The stability of the IgG is guaranteed for a period of 12 months from the date of receipt.
Target
Background	RAD18 is a crucial E3 ubiquitin-protein ligase that plays a significant role in repairing UV-damaged DNA after replication. This important postreplication repair function involves filling in gaps in the daughter strand of damaged DNA. RAD18 forms a complex with the E2 ubiquitin conjugating enzyme UBE2B, called the UBE2B-RAD18 ubiquitin ligase complex, which is essential for the mono-ubiquitination of DNA-related PCNA on 'Lys-164'. In addition to its ubiquitin ligase activity, RAD18 also has an affinity for ssDNA.
Celluar localization	Cytoskeleton;Nucleus;
UniPort	Q9NS91