Rabbit Anti-TNFR1 Polyclonal Antibody #abs130218

abs130218-50ug

1-2 Weeks

201

abs130218-100ug

1-2 Weeks

301

Description

The TNF-R1 receptor plays a crucial role in inducing apoptosis in response to TNFSF2/TNF-alpha and TNFSF1/lymphotoxin-alpha. Upon activation, the adapter molecule FADD recruits caspase-8 to the activated receptor, which leads to the formation of the death-inducing signaling complex and subsequent cascade of caspases. Apart from apoptosis, TNF-R1 also contributes to the induction of non-cytocidal TNF effects such as the activation of the acid sphingomyelinase and anti-viral state. TNF-induced homotrimerization of the receptor leads to the aggregation of death domains, which create a unique molecular interface that interacts specifically with TRADD's death domain.

Other names

TNF receptor 1, also known as TNFR1 or TNFRSF1a, is a protein that belongs to the tumor necrosis factor receptor superfamily. It is encoded by the genes CD120a, FPF, MGC19588, p55, p55-R, p60, TBP1, TBPI, TNF R, TNF R55, TNF-R1, TNF-RI, TNFAR, TNFR-I, TNFR1, TNFR55, TNFR60, TNFRI, TNFRSF1a, TNR1A_HUMAN, and tumor necrosis factor receptor type 1. Additionally, it is sometimes referred to as tumor necrosis factor receptor type I or tumor necrosis factor-binding protein 1. This receptor plays a significant role in cytokine signaling and is involved in the regulation of immune responses and inflammation.

Source

Rabbit

Specificity

TNFR1 Antibody detects endogenous levels of TNFR1

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilutions for IHC staining are in the range of 1:50 to 1:200, while for Western Blotting, it is suggested to use dilutions ranging from 1:500 to 1:2000. In the case of ELISA assays that use peptides, recommended dilutions for detection are typically within the range of 1:20000 to 1:40000. These dilution ratios may vary based on experimental conditions and the specificity of the antibody or peptide used for detection. Nonetheless, following these recommended dilutions can aid in achieving optimal results in experimental assays.

Immunogen

A synthesized peptide derived from human TNFR1

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To ensure maximum stability, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to note that it is best to avoid exposing the product to any repeated freeze/thaw cycles, as this could potentially compromise its quality and effectiveness. So, be sure to store the product in a cool and dry place, to prevent any possible degradation.

Storage buffer

Please note that the generated content is in complete contrast to the original text information:
The storage condition for Rabbit IgG is an icy environment of -20 °C. It is advisable to store it in phosphate buffered saline with a pH level of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This particular product has a shelf life of 12 months from the date of receipt, ensuring its stability and efficacy.

Target

Background

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

Posttranslational modification

The soluble form is produced from the membrane form by proteolytic processing.

Celluar localization

Extracellular region or secreted;Golgi apparatus;Mitochondrion;Plasma Membrane;

UniPort

P19438