Rabbit Anti-TIMP4 Polyclonal Antibody #abs130217

abs130217-50ug

1-2 Weeks

201

abs130217-100ug

1-2 Weeks

301

Description

TIMP4 is a powerful inhibitor of metalloproteinases, specifically collagenases. This protein binds to the catalytic zinc cofactor of these enzymes, causing them to become irreversibly inactivated. Known to act on several different MMPs, including MMP1, MMP2, MMP3, MMP7, and MMP9, TIMP4 belongs to the I35 (TIMP) family of protease inhibitors. By blocking the action of these enzymes, TIMP4 is an important regulatory protein in a variety of biological processes.

Other names

The protein known as Metalloproteinase inhibitor 4, also referred to as TIMP 4 or TIMP metallopeptidase inhibitor 4, plays a crucial role in inhibiting the activities of metalloproteinases. It is encoded by the TIMP4 gene and is commonly found in humans. TIMP-4, which stands for Tissue inhibitor of metalloproteinase 4, is a key regulator of extracellular matrix remodeling.
Timely expression of Timp4 is essential for maintaining proper tissue homeostasis. This protein functions by binding to active metalloproteinases, thus preventing them from degrading components of the extracellular matrix. As a result, the integrity and stability of tissues are maintained.
Research has indicated that alterations in TIMP4 levels or function can contribute to various pathological conditions, including cancer, cardiovascular diseases, and arthritis. Therefore, understanding the mechanisms underlying TIMP4 regulation and its interaction with other proteins is of great interest in the field of biomedicine.
The discovery and characterization of Timp4 have enhanced our knowledge of the intricate processes involved in tissue remodeling and repair. Continued investigation into its structure and function will undoubtedly provide valuable insights for the development of novel therapeutic approaches targeting metalloproteinase activity in various diseases.

Source

Rabbit

Specificity

Endogenous levels of total TIMP4 can be detected by utilizing an antibody specific to TIMP4. The TIMP4 antibody is able to accurately detect the presence of TIMP4 within various samples, allowing for further analysis and research to be conducted. By targeting endogenous levels of TIMP4, this antibody provides a valuable tool for studying the role and function of TIMP4 in a variety of biological processes.

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilutions for using this antibody in various applications are as follows: for Western blotting, use a dilution of 1:500 to 1:3000; for immunohistochemistry, use a dilution of 1:50 to 1:200; for immunofluorescence or immunocytochemistry, use a dilution of 1:100 to 1:500; and for ELISA involving peptides, use a dilution range of 1:20000 to 1:40000. These guidelines will likely yield optimal results when using this antibody in your experiments. It is important to note that slight variations in the optimal dilution may be necessary depending on the specific experimental conditions. Overall, this antibody has the potential to be a valuable tool in a variety of research applications.

Immunogen

A synthesized peptide derived from human TIMP4.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to perform peptide affinity chromatography, which helped purify the antiserum. The resulting product is highly effective and closely resembles the original substance.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain its quality, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to multiple freeze and thaw cycles.

Storage buffer

The storage condition for Rabbit IgG is in phosphate-buffered saline at pH 7.4. The solution contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at -20 °C. The product is stable for 12 months from the date of receipt.

Target

Background

Metalloproteinases like collagenases are effectively neutralized by complexes that bind to their catalytic zinc cofactor, leading to irreversible inactivation. This process targets MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9, ensuring their suppression.

Tissue specificity

Present in low levels in many other tissues and abundant in the heart.

Celluar localization

Extracellular region or secreted;

UniPort

Q99727