E-cadherin Antibody #abs130068

abs130068-50ug

In Stock

201

abs130068-100ug

In Stock

301

Description

CDH1 is an integral membrane protein belonging to the type I category. It plays a crucial role in cell adhesion and relies on calcium for its function. Specifically, it acts as a ligand for integrin alpha-E/beta-7 and is found in close proximity to DLG7 at areas of cell-to-cell contact in the intestinal epithelial cells.
CDH1 is anchored to actin microfilaments through its association with alpha-, beta-, and gamma-catenin. Under certain conditions such as apoptosis or an increase in calcium levels, sequential proteolysis occurs, leading to the relocation of CDH1 from cell-to-cell contact sites to the cytoplasm.
This protein is involved in the regulation of various mechanisms that govern cell adhesions, mobility, and proliferation of epithelial cells. However, defects in CDH1 can disrupt the cell-cell adhesion system, ultimately triggering the invasion and spread of cancer. It has been associated with several types of cancer, including gastric, breast, ovary, endometrium, and thyroid cancers, and is known to facilitate metastasis.
In summary, CDH1 is a critical calcium-dependent cell adhesion protein that interacts with integrin alpha-E/beta-7 and localizes to cell-to-cell contact sites in the intestinal epithelial cells. Its association with actin microfilaments through catenins ensures its structural stability. However, abnormalities in CDH1 function can lead to cancer invasion and metastasis in various organs.

Other names

Cadherin 1, also known as E-cadherin or epithelial calcium dependent adhesion protein, is a type 1 cadherin that plays a crucial role in maintaining cell-cell adhesion and epithelial integrity. It is a transmembrane protein that interacts with other cadherins in adjacent cells to form strong adhesive bonds. CD324 antigen, CAM 120/80, LCAM, liver cell adhesion molecule, CDHE, Uvomorulin, and CD 324 are all alternative names for this protein.
E-cadherin has been shown to be involved in various biological processes, including embryonic development, tissue homeostasis, and tumor suppression. Loss or aberrant expression of E-cadherin has been implicated in the progression of several types of cancers, including breast, prostate, and gastric cancers.
E-cadherin is encoded by the CDH1 gene located on chromosome 16q22.1. Mutations in this gene can lead to hereditary diffuse gastric cancer, a condition characterized by the development of multiple stomach tumors.
In summary, E-cadherin is a crucial protein for maintaining tissue integrity and preventing cancer, and alterations in its expression can have serious implications for human health.

Source

Rabbit

Specificity

The detection of endogenous levels of total E-cadherin can be achieved using an E-cadherin antibody.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Zebrafish;Rabbit;Pig;Dog;Horse;Sheep;Bovine

Application

Western blot (WB) is typically performed at dilutions ranging from 1:500 to 1:3000. Immunohistochemistry (IHC), on the other hand, requires dilutions between 1:50 and 1:200. For immunofluorescence and immunocytochemistry (IF/ICC), the recommended dilutions are between 1:100 and 1:500. In ELISA assays using peptides, a dilution range of 1:20000 to 1:40000 is commonly used. It is important to adhere to these dilution guidelines in order to achieve reliable and accurate results in your experiments.

Immunogen

A peptide synthesized from human E-cadherin is now available. This peptide is derived from the human protein and is highly similar to the original sequence. It has been synthesized in a lab and can be used for various applications. This peptide may be useful for studying the function and structure of E-cadherin and for developing potential therapies. Additionally, this synthesized peptide may provide insights into the mechanisms of cell adhesion and migration, which are important for understanding processes such as embryonic development and cancer metastasis.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum. A highly similar content can be generated by rearranging the provided information as follows:

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of your product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze and thaw cycles, as this can compromise its quality. Preserve the integrity of your product by following these storage guidelines.

Storage buffer

This solution contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, along with 150mM NaCl, 0.02% sodium azide, and 50% glycerol for stabilization. It is recommended to store this solution at a temperature of -20 °C for optimal longevity, with 12 months of stability guaranteed from the date of receipt.

Target

Background

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Tissue specificity

Non-neural epithelial tissues.

Posttranslational modification

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.N-glycosylation at Asn-637 is essential for expression, folding and trafficking.Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

Celluar localization

Cytoskeleton;Endosome;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;

UniPort

P12830