Rabbit Anti-Fos Polyclonal Antibody #abs130069

abs130069-50ug

1-2 Weeks

201

abs130069-100ug

1-2 Weeks

301

Description

FOS is a transcription factor belonging to the bZIP family with proto-oncogene characteristics. It forms a complex with proteins of the JUN family, leading to the formation of the AP-1 transcription factor. Its primary role is to regulate cell proliferation, differentiation, and transformation. Interestingly, FOS expression has also been linked with apoptotic cell death in certain cases. The expression of this protein increases upon exposure to several stimuli comprising growth factors, cytokines, neurotransmitters, polypeptide hormones, stress, and cell injury.

Other names

Activator protein 1, also known as AP-1, is a transcription factor that plays a crucial role in cellular processes. It is encoded by the c-FOS gene, also known as the cellular oncogene c-FOS or FBJ murine osteosarcoma viral (v-fos) oncogene homolog. FOS protein, also referred to as FOS_HUMAN or p55, is the protein product of this gene.
FOS is an important proto-oncogene that has been widely studied for its involvement in cell growth, differentiation, and transformation. It functions as a transcription factor by binding to specific DNA sequences and regulating the expression of target genes. Through its interaction with other proteins, FOS can form the heterodimeric AP-1 complex, which is involved in the regulation of numerous cellular processes.
The FBJ murine osteosarcoma viral v-fos oncogene homolog, also known as FBJ Osteosarcoma Virus, is a viral oncogene that shares homology with the cellular proto-oncogene FOS. It has been extensively studied due to its ability to induce the formation of tumors in experimental models.
Overall, the FOS protein and its associated genes have been the subject of extensive research aimed at understanding their role in normal cellular processes and their contribution to oncogenesis.

Source

Rabbit

Specificity

The total Fos levels, which are naturally occurring, can be detected using the Fos antibody.

Species Reactivity

Human;Mouse;Rat

Application

1:500~1:3000 for Western blot (WB), 1:50~1:200 for immunohistochemistry (IHC), 1:100-1:500 for immunofluorescence/immunocytochemistry (IF/ICC), and 1:20000-1:40000 for ELISA (peptide). I hope this rearranged information is helpful.

Immunogen

A synthesized peptide derived from human Fos.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum. With this method, a highly purified form of the antiserum was obtained.

Clonality

Polyclonal Antibody

Stability & Storage

It is recommended to store the item at a temperature of -20 °C for a period of one year. It is important to avoid any instances of repetitive freeze/thaw cycles. It is advisable to adhere strictly to these storage guidelines to ensure the longevity of the product.

Storage buffer

This rabbit IgG is suspended in a buffer of phosphate buffered saline at a pH of 7.4, along with 150mM of NaCl and 0.02% sodium azide. It also contains 50% glycerol which acts as a good stabilizer. To ensure that the product remains stable, it’s recommended to store it at -20 °C. The IgG is highly stable and can be preserved for up to 12 months, starting from the date of receipt.

Target

Background

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Tissue specificity

Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.

Posttranslational modification

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.

Celluar localization

Cytosol;Endoplasmic reticulum;Nucleus;

UniPort

P01100