Rabbit Anti-CTH Polyclonal Antibody #abs128251

abs128251-50ul

In Stock

201

abs128251-100ul

In Stock

301

Other names

Cystathionine gamma lyase, also known as cystathionine gamma-lyase or CSE, is an enzyme that is responsible for catalyzing the breakdown of cystathionine into cysteine and alpha-ketobutyrate. This enzyme also plays an important role in the biosynthesis of hydrogen sulfide, which is an important signaling molecule in the body. CSE is also known as cysteine desulfhydrase, gamma cystathionase, or homoserine dehydratase, among other names. It is encoded by the CTH gene and is found in various tissues throughout the body. Dysfunction of CSE has been linked to a number of different diseases and conditions, including cardiovascular disease, diabetes, and cancer. However, further research is needed in order to fully understand the role that this enzyme plays in human health and disease.

Source

Rabbit

Specificity

CTH

Species Reactivity

Human;Mouse;Rat;Dog;Cow;Rabbit;

Application

The dilution factors for WB, ELISA, IHC-F, and IF are as follows: WB can be diluted from 1:500 to 1:2000, ELISA from 1:500 to 1:1000, IHC-F from 1:400 to 1:800, and IF from 1:50 to 1:200. It is important to note that when working with paraffin sections, antigen retrieval is required for optimal results.

Immunogen

A synthetic peptide, conjugated with KLH and derived from the human protein CTH/CSE/Cystathionase, has been generated. The peptide comprises amino acid residues ranging from 51 to 150 out of a total of 405 residues present in the protein. To create similar content, one can rearrange the above information to say that a KLH-conjugated synthetic peptide has been developed based on human CTH/CSE/Cystathionase. This peptide is composed of residues 51 to 150 out of a total of 405 residues present in the protein.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

The storage temperature for one year should be maintained at -20 °C. It is important to prevent repeated freezing and thawing cycles.

Storage buffer

A solution containing 1% BSA, 0.03% Proclin300, and 50% Glycerol was prepared in 0.01M TBS at pH7.4.

Research area

Cell Biological Signal Transduction in Tumor Cardiovascular

Target

Background

This enzyme plays a crucial role in the transsulfuration pathway, serving as the catalyst for the final step in the conversion of methionine to cysteine. It displays a wide range of substrate acceptance, exhibiting broad specificity. Its primary function is to transform cystathionine into cysteine, ammonia, and 2-oxobutanoate. Interestingly, it also has the capability to convert two cysteine molecules into lanthionine and hydrogen sulfide. Moreover, it can accept homocysteine as a substrate, although its specificity depends on the levels of the endogenous substrates present. One of its key contributions lies in its ability to generate hydrogen sulfide (H2S), an important signaling molecule involved in the regulation of blood pressure.
Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.

Celluar localization

Cytoplasm

UniPort

P32929