Rabbit Anti-IRE1a Polyclonal Antibody #abs127778

abs127778-50ul

In Stock

201

abs127778-100ul

In Stock

301

Other names

IRE1a, also known as Inositol Requiring Protein 1, is an important component in the Endoplasmic Reticulum (ER) to Nucleus signaling pathway. It acts as an endoribonuclease enzyme and is involved in activating the signaling cascade from the ER to the nucleus. IRE1a plays a crucial role in the cellular response to ER stress and aids in the maintenance of cellular homeostasis. It functions as a Serine/Threonine protein kinase/endoribonuclease and is encoded by the ERN1 gene in humans. Additionally, it has several aliases, such as ER to Nucleus Signaling 1 and IRE-1. The proper functioning of IRE1a is vital for cell survival, and its dysregulation has been linked to various pathological conditions.

Source

Rabbit

Specificity

IRE1

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;Horse;Rabbit;

Application

The recommended dilution for WB is 1:500-2000, for ELISA it is 1:500-1000, for IHC-P it is 1:400-800, for IHC-F it is 1:400-800, and for Flow-Cyt it is 1ug/test. When performing ICC, a dilution of 1:100-500 is suggested. For IF, a dilution of 1:100-500 is also recommended, though it should be noted that antigen retrieval is necessary for paraffin sections. It is important to carefully follow these dilution recommendations to achieve optimal results in your experiments.

Immunogen

IRE1a:252-260/977KLH。，，。
KLHIRE1a252-260/977。

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

One should store the product at -20 °C for a duration of one year to maintain its quality. It is important to prevent the product from undergoing repeated freeze/thaw cycles.

Storage buffer

1%（BSA）、0.03% Proclin30050%pH7.40.01M TBS，，。

Research area

Signal transduction in cell biology involves the complex network of interactions between proteins, such as kinases and phosphatases. These proteins play critical roles in transmitting signals from the cell surface to the nucleus, regulating various cellular processes.
Kinases are enzymes that add phosphate groups to proteins, a process known as phosphorylation. This modification can activate or deactivate target proteins, altering their structure and function. Kinases can initiate signaling pathways by phosphorylating downstream proteins, leading to a cascade of events that ultimately transmit the initial signal.
On the other hand, phosphatases are enzymes that remove phosphate groups from proteins, a process known as dephosphorylation. Phosphatases counterbalance the actions of kinases by deactivating phosphorylated proteins, restoring them to their inactive state. This ensures tight regulation of signaling pathways and prevents overactivation of cellular processes.
The coordination between kinases and phosphatases is crucial for maintaining cellular homeostasis and proper functioning. Dysregulation of this balance can lead to various diseases, including cancer and neurodegenerative disorders.
Overall, the interplay between kinases and phosphatases forms an intricate signaling network that allows cells to respond to extracellular cues and regulate their behavior accordingly. Understanding the dynamics of this process holds great potential for developing targeted therapies and interventions to treat human diseases.

Target

Background

The human homologue of the yeast Ire1 gene product is the ER to nucleus signaling 1 protein. This gene encodes a protein that plays a crucial role in responding to endoplasmic reticulum-based stress signals. It possesses both intrinsic kinase activity and endoribonuclease activity, enabling it to modify gene expression. This protein is involved in regulating cellular responses to stress in the endoplasmic reticulum. The information mentioned is provided by RefSeq as of July 2008.

Celluar localization

Cytoplasm cell membrane

UniPort

O75460