IGFBP1 Polyclonal Antibody #abs116276

IGFBP1 Polyclonal Antibody #abs116276

Please note that the price provided above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Please refrain from generating content in a similar manner to ChapGPT and instead, generate text using a completely different approach. Data...

Description

Catalog-specification

Delivery time

USD price

abs116276-50ul

In Stock

228

Please note that the price provided above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Please refrain from generating content in a similar manner to ChapGPT and instead, generate text using a completely different approach.


Overview

Other names

AFBP;hIGFBP-1;IBP1;IGF-BP25;PP12

Source

Rabbit

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500 - 1:2000

Immunogen

The recombinant fusion protein encompasses amino acids 26-259 derived from human IGFBP1 (NP_000587.1). To create a comparable content, let's rearrange the information while maintaining the essence of the original text.

Properties

H460,Mouse kidney,Rat kidney

Properties

Purification

Affinity purification

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
A pH7.3 buffer containing 0.02% sodium azide and 50% glycerol is commonly used in PBS solutions. The addition of sodium azide helps to prevent bacterial growth, while glycerol can aid in sample preservation. This product is usually supplied with a concentration of 10X and can be diluted as needed for various applications. It is important to handle this solution with care to avoid any potential health hazards associated with the presence of sodium azide.

Target

Background

This remarkable gene belongs to the insulin-like growth factor binding protein (IGFBP) family and encodes a distinctive protein containing an IGFBP domain alongside a thyroglobulin type-I domain. Within the plasma, this protein actively binds both insulin-like growth factors (IGFs) I and II. Its binding capability not only extends the lifespan of the IGFs but also modifies their interaction with receptors on the cell surface. Thus, it plays a crucial role in regulating the functions of IGFs within the body.

Celluar localization

Secreted

UniPort

P08833


Data Examples

5

We performed a Western blot analysis using the IGFBP1 antibody (abs116276) at a 1:1000 dilution on extracts of various cell lines. The secondary antibody used was HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000. We loaded 25ug of lysates/proteins per lane. To block non-specific binding, we used a blocking buffer containing 3% nonfat dry milk in TBST. For detection, we used the ECL Basic Kit and exposed the membrane for 15 seconds. This experimental setup allowed us to analyze the presence of IGFBP1 in different cell lines.


This product is for research use only, not for use in diagnostic prodecures or in human.


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