Rabbit Anti-IL-13 Polyclonal Antibody #abs120028

abs120028-50ul

In Stock

201

abs120028-100ul

In Stock

301

Other names

IL13 (Interleukin 13) is also known as BHR1, ALRH, or T-cell activation protein P600. It is encoded by the MGC116786, MGC116788, and MGC116789 genes. IL13 plays a crucial role in regulating immune responses and inflammation. It functions by binding to its specific receptor and activating various downstream signaling pathways. IL13 has been implicated in a wide range of diseases, including asthma, atopic dermatitis, and cancer. Additionally, IL13 has been extensively studied as a potential therapeutic target for the treatment of these conditions. Understanding the mechanisms and functions of IL13 is vital for developing effective therapies and improving patient outcomes.

Source

Rabbit

Specificity

IL-13

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;Sheep;

Application

。WB（），1:5001:2000；ELISA（）1:5001:1000；IHC-P（-）IHC-F（-）1:4001:800；IF（）1:1001:500。，IHC-P，。。

Immunogen

KLHIL-13（45-56aa）：45-56/131（mo）。，。

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

It is advisable to place the item in a -20 °C storage facility for up to one year. It is important to avoid subjecting it to numerous freeze/thaw cycles to maintain its quality. To generate a similar content, the information can be rephrased as follows: To preserve the item's quality, it's recommended to store it at -20 °C for a maximum of one year, while ensuring to avoid repeating the process of freezing and thawing.

Storage buffer

A solution of 0.01M TBS at pH7.4 has been prepared and fortified with 1% Bovine Serum Albumin (BSA) for protein stabilization. In addition, 0.03% Proclin300 has been added as a preservative to prevent bacterial growth. Finally, 50% glycerol has been introduced to prevent freezing and thawing damage during storage.

Research area

Tumor Cell Biology Immunology

Target

Background

The discovery of human IL13 was made possible through the use of various cDNA libraries. One such library was an anti CD28 activated human peripheral blood mononuclear cell cDNA library, which revealed IL13 to be an induction-specific cytokine. Additionally, murine IL13 (P600) was utilized as a probe to isolate IL13 from cDNA libraries of human T cell clones. This pleiotropic cytokine is produced by activated Th0, Th1-like, Th2-like, and CD8 T cells. The gene for human IL13 is located on chromosome 5 and is closely linked to other genes responsible for the production of cytokines including IL3, IL4, IL5, and GMCSF.
IL13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity.
Human and mouse interleukin 13 share approximately 58% amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30% sequence homology and have similar biological functions.

Celluar localization

Secreted protein

UniPort

P20109