Rabbit Anti-MDR1 Polyclonal Antibody #abs120030

abs120030-50ul

In Stock

201

abs120030-100ul

In Stock

301

Other names

ATP-binding cassette sub-family B (MDR/TAP), member 1, also known as P-Glycoprotein or Multi Drug Resistance Associated Protein, is a protein encoded by the ABCB1 gene in humans. This protein, also referred to as CD243 or CD243 antigen, plays a crucial role in drug resistance. It is involved in the transport of various drugs out of cells, thereby reducing their efficacy in treating diseases.
P-Glycoprotein, with its alternative names including CLCS, Multidrug resistance protein 1, P glycoprotein 1, p-GP, gp170, PGY1, and MDR1_HUMAN, is a highly important transporter protein. It is responsible for the efflux of a wide range of drugs from cells, making it a key player in the development of drug resistance.
The ABCB1 gene encodes P-Glycoprotein, which belongs to the ATP-binding cassette (ABC) transporter superfamily. This protein utilizes energy from ATP hydrolysis to pump drugs out of cells, preventing them from reaching their intended targets. By doing so, it confers resistance to various chemotherapeutic agents, making cancer treatment more challenging.
Understanding the structure and function of P-Glycoprotein is essential in developing strategies to overcome drug resistance. Targeting this protein and inhibiting its activity could enhance drug efficacy and improve patient outcomes, particularly in the field of cancer treatment. Advancements in this area of research hold promise for the development of more effective therapies against various diseases.

Source

Rabbit

Specificity

MDR1

Species Reactivity

Human;Mouse;Rat;

Application

The recommended dilution ranges for using this antibody in various applications are as follows: 1:500-2000 for Western blotting, 1:500-1000 for ELISA assays, 1:400-800 for both IHC-P and IHC-F, and 1μg per test for Flow cytometry. For immunofluorescence microscopy, the recommended dilution range is 1:100-500. Keep in mind that antigen retrieval is required for paraffin sections. To achieve optimal results, it is important to carefully consider the appropriate dilution range for your specific experiment and adjust accordingly.

Immunogen

KLHMDR1:21-100/1272。，。

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Please ensure that the product is stored at -20 °C for one year and avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

A solution of 0.01M TBS with a pH of 7.4, containing 1% BSA, 0.03% Proclin300, and 50% glycerol, is what we have here. We can rearrange the content to say that this is a mixture of tris-buffered saline with a pH of 7.4, made up of 1% bovine serum albumin, 0.03% Proclin300, and 50% glycerol.

Research area

In the field of biology, there are various disciplines that are worth exploring. One of the most fascinating areas of study is the cardiovascular system, which includes the heart and blood vessels. Another crucial area of research is tumor biology, which focuses on the growth and development of tumors in different parts of the body. Additionally, immunology is a critical discipline that deals with the complex interactions between the immune system and various pathogens.
One important aspect of all these fields is the role of signal transduction, which refers to the multiple steps that are involved in the communication between cells. Transcription regulators are also important since they help to control gene expression, which can have a significant impact on diverse cellular processes. By studying these different areas of biology, researchers can gain insights into how different systems in the body work together and how to develop new treatments for various diseases.

Target

Background

P Glycoprotein, an ATP-dependent drug efflux pump, is primarily found in cells that perform secretory and excretory functions. These malignant cells exhibit broad substrate specificity and play a vital role in the normal secretion of metabolites and ingested chemicals into bile, urine, and the intestinal tract. The kidney's proximal tubules, liver's bile canaliculi, adrenal gland, and intestine exhibit the highest expression of P Glycoprotein. However, P Glycoprotein levels significantly increase in multidrug-resistant cell lines, as well as in various tumors such as colorectal, endometrial, ovarian, and breast tumors. It is also found in sarcomas, leukemias, and lymphomas.

Celluar localization

Cell membrane

UniPort

P21447