Rabbit Anti-Vimentin Polyclonal Antibody #abs130228

abs130228-50ug

1-2 Weeks

201

abs130228-100ug

1-2 Weeks

301

Description

Mesenchymal tissue expresses vimentin as an intermediate filament protein, which is specific to this type of tissue. In muscle tissue, the subunit specific for intermediate filaments is desmin. Overall, intermediate filament proteins are expressed in a manner that is specific to particular tissues.

Other names

CTRCT30、Epididymis luminal protein 113、FLJ36605、HEL113、VIMVIME_HUMAN。Vimentin。。Vimentin，。, 。

Source

Rabbit

Specificity

The Vimentin antibody has the capability to detect the endogenous levels of total Vimentin, which is critical for various biological processes. This antibody can identify the protein under its natural form without interfering with its function, making it a useful tool in both research and clinical settings. By detecting Vimentin, it is possible to better understand its involvement in normal and pathological cellular mechanisms.

Species Reactivity

Human;Mouse;Rat

Application

For Western blotting, dilute the primary antibody at a range of 1:500 to 1:3000. When performing immunohistochemistry, a dilution of 1:50 to 1:200 is appropriate for the primary antibody. For immunofluorescence or immunocytochemistry, the primary antibody should be diluted to 1:100-1:500. Finally, for ELISA assays using peptides, a dilution of 1:20000 to 1:40000 is optimal for the primary antibody. Remember to adjust the dilutions as needed for the specific assay and target of interest.

Immunogen

A synthesized peptide derived from human Vimentin.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To keep the product in good condition, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can cause damage and degradation over time. It is highly advisable to adhere to these storage guidelines in order to ensure the product maintains its efficacy for as long as possible.

Storage buffer

This Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol, and should be kept at a temperature of -20 °C. It is stable for a period of 12 months from the time of receipt.

Target

Background

Vimentins are a type of class-III intermediate filaments that can be found in numerous non-epithelial cells, especially in those mesenchymal cells that are responsible for supporting and connecting the various organs and tissues in the body. These intermediate filaments are known to be attached to the nucleus, endoplasmic reticulum, and mitochondria, often forming lateral or terminal connections.

Tissue specificity

Fibrin, a protein highly expressed in fibroblasts, also shows some expression in T- and B-lymphocytes. Interestingly, this protein is rarely or not expressed in Burkitt's lymphoma cell lines. On the other hand, many hormone-independent mammary carcinoma cell lines show a significant level of Fibrin expression.

Posttranslational modification

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Peroxisome;Plasma Membrane;

UniPort

P08670