Rabbit Anti-ChRM2 Polyclonal Antibody #abs119939

abs119939-50ul

In Stock

201

abs119939-100ul

In Stock

301

Other names

The receptor known as Muscarinic Acetylcholine Receptor 2, also referred to as 7TM receptor, is a crucial component involved in the acetylcholine signaling pathway. It is responsible for mediating the effects of acetylcholine in various cells and tissues. With the human gene being denoted as CHRM2, this receptor plays a significant role in cardiac function, particularly in the isoform labeled as a. Other aliases for this receptor include ACM2_HUMAN, Acm2, Cholinergic receptor muscarinic 2, and Cholinergic receptor, muscarinic 2a. It is worth noting that CHRM2 is also known by several other names such as CM2, FLJ43243, M2, HM2, MGC120006, MGC120007, Muscarinic acetylcholine receptor M2, and Muscarinic M2 receptor. Overall, the Muscarinic Acetylcholine Receptor 2 represents a crucial molecular target involved in cholinergic signaling pathways with diverse implications in physiological and pathological processes.

Source

Rabbit

Specificity

ChRM2

Species Reactivity

Human;Mouse;Rat;Chicken;Dog;Pig;Cow;

Application

The dilutions for WB are 1:500-2000, for ELISA are 1:500-1000, for IHC-P are 1:400-800, for IHC-F are 1:400-800, for Flow-Cyt are 1μg/Test, for ICC are 1:100-500, and for IF are 1:100-500 (with antigen retrieval required for paraffin sections). Please note that the following text is generated based on the original information but rearranged for a different presentation:
- For Western Blotting (WB), the recommended dilutions range from 1:500 to 2000.
- ELISA (Enzyme-Linked Immunosorbent Assay) should be performed using dilutions of 1:500 to 1000.
- When conducting Immunohistochemistry on paraffin-embedded sections (IHC-P), it is advised to use dilutions ranging from 1:400 to 800.
- For Immunohistochemistry on frozen sections (IHC-F), the recommended dilutions are 1:400 to 800.
- Flow Cytometry (Flow-Cyt) requires 1μg of the test sample.
- Immunocytochemistry (ICC) should be performed using dilutions of 1:100 to 500.
- Similarly, Immunofluorescence (IF) should be conducted using dilutions of 1:100 to 500. Please note that antigen retrieval is necessary when working with paraffin sections.

Immunogen

A synthetic peptide derived from the 168-192 amino acid region of human ChRM2 (168-250/466) has been conjugated with KLH. If you rearrange this information, it can be stated that KLH-conjugated synthetic peptide has been developed from the human ChRM2 (168-192aa) region, precisely from 168-250/466 amino acid sequence.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To maintain quality, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze and thaw cycles.

Storage buffer

In a solution of pH7.4 TBS with a concentration of 0.01M, there is a 1% presence of BSA along with 0.03% Proclin300 and 50% Glycerol. This composition is aimed at ensuring that the solution remains stable and effective in carrying out its intended function. The presence of these components ensures that the solution is not affected by external factors and can maintain its structure over a prolonged period. With this composition, the solution can be used effectively for various applications.

Research area

Neurobiology cell membrane receptor

Target

Background

Muscarinic cholinergic receptors, which are part of the G protein-coupled receptor family, exhibit functional diversity based on their interaction with acetylcholine. These receptors play a role in various cellular responses including the inhibition of adenylate cyclase, degradation of phosphoinositide, and mediation of potassium channels. They have a significant impact on the effects of acetylcholine in both the central and peripheral nervous systems. In particular, muscarinic cholinergic receptor 2 is involved in regulating bradycardia (a slower heart rate) and reducing cardiac contractility. This gene has been found to have multiple alternatively spliced transcript variants. This information is based on the findings provided by RefSeq in July 2008.

Celluar localization

Cell membrane

UniPort

P08172