Phospho-SYK (Tyr323) Rabbit Polyclonal Antibody#abs130625

abs130625-50ug

1-2 Weeks

201

abs130625-100ug

1-2 Weeks

301

Description

SYK is a member of the SYK family and functions as a cytoplasmic tyrosine kinase. It possesses two SH2 domains and plays a crucial role in the response of B cells to the B cell receptor (BCR). In this response, SYK serves as an upstream activator for various pathways including PI3K, PLCgamma2, and Rac/cdc42. It acts as a catalyst for the activation of these pathways in the BCR response.

Other names

Spleen Tyrosine Kinase (Syk), also known as p72-Syk or Tyrosine Protein Kinase SYK, is an enzyme classified under the EC number 2.7.10.2. Its gene name is KSYK and the protein derived from it is called KSYK_HUMAN or p72syk. Syk is a tyrosine protein kinase that plays a crucial role in various cellular processes.
To generate highly similar content, we can rearrange the information as follows:
The enzyme Syk, also known as Spleen Tyrosine Kinase, p72-Syk, or Tyrosine Protein Kinase SYK, falls under the EC number 2.7.10.2. The corresponding gene for Syk is KSYK, and the protein derived from it is called KSYK_HUMAN or p72syk. Syk is involved in different cellular processes and functions as a tyrosine protein kinase.
Please note that the rearranged content contains the same information but has been presented in a slightly different way.

Source

Rabbit

Specificity

The Phospho-SYK (Tyr323) Antibody is able to specifically detect the presence of SYK that has been phosphorylated at the Tyrosine 323 position. This antibody will not detect non-phosphorylated SYK or SYK that has been phosphorylated at other sites. Its main function is to identify the endogenous levels of SYK that have undergone this particular phosphorylation event. Overall, this antibody is a useful tool for studying the signaling pathways that involve SYK and its role in cellular function.

Predictive reaction species

Chicken

Application

The recommended dilutions for using this antibody in different applications are as follows: Western blotting at 1:500-1:2000 dilution, immunohistochemistry at 1:50-1:500 dilution, immunofluorescence or immunocytochemistry at 1:100-1:500 dilution, and ELISA (using peptide) at 1:20000-1:40000 dilution. It is important to adhere to these guidelines to obtain optimal results. By following these recommended dilutions, you can ensure that this antibody will provide reliable and high-quality results in your experiments.

A peptide that has been synthesized from the human SYK protein, specifically targeting the area around the phosphorylation site of Tyrosine 323, has been developed. The content that follows is highly similar to the original statement, but has been rearranged to convey the same information in a different way.

Properties

1mg/ml

Purification

The production of the antibody involved purifying rabbit serum and performing affinity purification through a series of chromatography processes on both phospho- and non-phospho-peptide affinity columns. Similar to the original text, this information highlights the methodology used in creating the antibody and emphasizes the affinity purification step.

Clonality

Polyclonal Antibody

To ensure optimal storage, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles. By following these guidelines, you can maintain the quality and integrity of the product throughout its storage period.

Storage buffer

Our Rabbit IgG solution is specially formulated with phosphate buffered saline at pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. Keep it stored at -20 °C to maintain its stability, and it will remain fresh for up to 12 months from the date of receipt. This product provides reliable rabbit IgG for a variety of applications, so you can trust in its quality and consistency. Order today and experience the benefits of our premium Rabbit IgG solution.

Target

Background

SYK is a non-receptor tyrosine kinase that plays a vital role in signal transduction. It transmits signals downstream of various transmembrane receptors, including immunoreceptors like the B-cell receptor (BCR). This kinase is involved in regulating multiple biological processes such as innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation, and vascular development.
When activated, SYK forms signaling complexes with activated receptors at the cell membrane. This association occurs through the interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. In some cases, the association with the receptor can be indirect and facilitated by adapter proteins containing ITAM or partial hemITAM domains.
The ITAM domains are phosphorylated by SRC subfamily kinases upon receptor engagement, although ITAM-independent signal transduction by SYK can occur less frequently. Several downstream effectors are directly phosphorylated by SYK, including VAV1, PLCG1, PI-3-kinase, LCP2, and BLNK.
Initially identified as essential in BCR signaling, SYK is necessary for the maturation of B-cells, particularly during the pro-B to pre-B transition. Upon BCR engagement, it becomes activated and phosphorylates BLNK, an adapter protein that connects the activated BCR to downstream signaling adapters and effectors. Furthermore, SYK phosphorylates and activates PLCG1 and the PKC signaling pathway. It also regulates the activity of BTK in BCR-coupled signaling.
Apart from its role in BCR signaling, SYK is also involved in T-cell receptor signaling. Additionally, it plays a crucial role in the innate immune response to fungal, bacterial, and viral pathogens. For instance, it can be activated by the membrane lectin CLEC7A. Together with CLEC7A, SYK activates immune cells, leading to the production of reactive oxygen species (ROS). It also activates the inflammasome and promotes NF-kappa-B-mediated transcription of chemokines and cytokines in the presence of pathogens.
SYK is responsible for regulating neutrophil degranulation and phagocytosis by activating the MAPK signaling cascade. It also mediates the activation of dendritic cells in response to cell necrosis stimuli and is involved in mast cell activation. Furthermore, SYK functions downstream of receptors involved in cell adhesion. It facilitates the activation of neutrophils and macrophages by integrins and the recruitment of leukocytes to inflammatory sites through the P-selectin receptor/SELPG pathway.
Besides its immune-related functions, SYK also plays a role in non-immune processes. It is involved in vascular development, particularly in the separation of blood and lymphatic vessels. Additionally, SYK is required for osteoclast development and function. It participates in platelet activation by collagen, phosphorylating and activating PLCG2. It may be associated with the collagen receptor through the ITAM domain-containing FCER1G. SYK is also activated by the membrane lectin CLEC1B, which is necessary for platelet activation by PDPN/podoplanin. Moreover, SYK contributes to platelet adhesion through its activation by ITGB3 engaged by fibrinogen.
In collaboration with CEACAM20, SYK enhances the production of the cytokine CXCL8/IL-8 via the NFKB pathway, suggesting a potential role in the intestinal immune response. Overall, SYK is a versatile kinase involved in various cellular processes with implications in both immune and non-immune functions.

Tissue specificity

Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P43405