Rabbit Anti-FGFR2 Polyclonal Antibody #abs130096

abs130096-50ug

1-2 Weeks

201

abs130096-100ug

1-2 Weeks

301

Description

FGFR2 is a receptor tyrosine kinase that belongs to the FGFR family and plays a crucial role in binding fibroblast growth factor (FGF). It is known to be associated with various bone malformations and craniosynostotic syndromes, and mutations in this gene can lead to defects in the development of facial and limb structures. Syndromes like Crouzon syndrome, Beare-Stevenson cutis gyrata syndrome, Pfeiffer syndrome, Apert syndrome, and Jackson-Weiss syndrome have all been linked to mutations in this gene. Somatic mutations in this gene have also been observed in gastric cancer.
Interestingly, FGFR2 is amplified in gastric, breast, and a few B cell cancers, although it is deleted in glioblastoma. Researchers have identified around twenty different splice-variant isoforms of this gene, highlighting its complexity and importance in various physiological processes.
In summary, FGFR2 is a crucial protein that plays a critical role in bone development and maintenance. Mutations in this gene can lead to numerous syndromes, and changes in its expression have been linked to several different cancers.

Other names

Here are a few examples of rearranged content:
1. The BEK fibroblast growth factor receptor, also known as FGFR2, is a protein tyrosine kinase that plays a crucial role in cell growth and differentiation. It is expressed in bacteria and is involved in various developmental processes, including craniofacial development.
2. CD332 antigen, also known as FGFR2, is a receptor-like protein tyrosine kinase that is expressed in the skin and plays a key role in keratinocyte growth. It is involved in a number of signaling pathways, such as the MAPK and PI3K/Akt pathways, and is associated with Jackson Weiss syndrome and craniosynostosis.
3. FGFR-2, also known as KGFR or K-sam, is a hydroxyaryl protein kinase that is expressed in a variety of tissues and is involved in many physiological processes. It interacts with several different fibroblast growth factors (FGFs) and modulates their signaling, leading to various cellular responses.
4. The soluble FGFR4 variant 4, or TK25, is a splice variant of the FGFR4 gene that lacks the transmembrane domain. It is able to bind to FGFs and inhibit their signaling, which may have implications in tumor growth and metastasis.
5. CEK3, also known as ECT1 or CFD1, is a bacterial-expressed kinase that is involved in the regulation of cell cycle progression and DNA repair. It is a member of the Cdc7/Dbf4 kinase family and is highly conserved across different species.
Note: These examples are not intended to be used as-is, but rather as inspiration for generating unique content based on the original text.

Source

Rabbit

Specificity

The FGFR2 antibody is capable of detecting the complete levels of endogenous FGFR2. This means that the antibody is designed to recognize and bind to all forms of the protein that naturally occur in cells. With this antibody, researchers can accurately measure the total amount of FGFR2 present in a sample of cells or tissues. This information can be used to understand the role of FGFR2 in various biological processes and to develop new therapies that target this protein.

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilution for Western blotting is between 1:500 to 1:3000, while for immunohistochemistry, it is suggested to use a dilution ratio ranging from 1:50 to 1:200. For ELISA using peptide, the suggested dilution range is 1:20000 to 1:40000. It is important to note that these dilution ratios may vary based on the specific experiment and sample being used. Thus, it is necessary to optimize the dilution ratio for each individual experiment to ensure optimal results.

Immunogen

A synthesized peptide derived from human FGFR2.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the purification of the antiserum was accomplished through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to note that repeated freeze/thaw cycles should be avoided to prevent any possible damage to the product. You can rest easy knowing that your product will be well-preserved if these storage guidelines are followed properly.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be stored at -20°C in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that the stability of the product is guaranteed for 12 months from the date of receipt.

Target

Background

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.

Posttranslational modification

Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-769 is essential for interaction with PLCG1.N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.Ubiquitinated. FGFR2 is rapidly ubiquitinated after autophosphorylation, leading to internalization and degradation. Subject to degradation both in lysosomes and by the proteasome.

Celluar localization

Extracellular region or secreted;Golgi apparatus;Nucleus;Plasma Membrane;

UniPort

P21802