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Rabbit Anti-Collagen I Polyclonal Antibody #abs130071

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Description

Catalog-specification	Delivery time	USD price
abs130071-50ug	1-2 Weeks	201
abs130071-100ug	1-2 Weeks	301

Overview
Description	The fibrillar collagen found in most connective tissues is composed of chains encoded by a specific gene. This particular gene is responsible for producing one of the chains for type I collagen. Mutations in this gene have been linked to various conditions including osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. It is worth noting that the symptoms associated with mutations in this gene are typically milder compared to mutations in the gene for alpha-1 type I collagen. This is because alpha-2 collagen is less abundant in the body. Interestingly, multiple messages can be generated by this gene due to the presence of multiple polyadenylation signals. This characteristic is shared among most of the other collagen genes.
Other names	Type I collagen, also known as alpha 1 type I collagen, is a protein found in the human body. It is composed of two chains, alpha 1 and alpha 2. The alpha 2 chain is initially known as alpha 2 type I procollagen or alpha 2(I) procollagen. In its mature form, it is referred to as alpha 2(I)-collagen. The genetic codes for alpha 1 type I collagen and alpha 2 type I collagen are CO1A1_HUMAN and COL1A1/COL1A2, respectively. These collagens are crucial components of various tissues like skin, tendon, and bone. Other names used for alpha 1 type I collagen include collagen alpha 1 chain type I, Collagen alpha-1(I) chain, and collagen alpha-1(I) chain preproprotein. Similarly, alpha 2 type I collagen is also known as Collagen I alpha 1 polypeptide and Collagen I alpha 2 polypeptide. In some medical scenarios, alpha 1 type I collagen is referred to as collagen of skin, tendon and bone, alpha-1 chain. On the other hand, alpha 2 type I collagen is referred to as collagen of skin, tendon and bone, alpha-2 chain. The genes associated with these collagens are also involved in certain medical conditions. For example, mutations in the COL1A1 gene lead to Osteogenesis Imperfecta type I (OI1) or Osteogenesis Imperfecta type II (OI2), while mutations in the COL1A2 gene cause Osteogenesis Imperfecta type III (OI3) or Osteogenesis Imperfecta type IV (OI4). Collectively, alpha 1 type I collagen and alpha 2 type I collagen are known as type I procollagen, or pro-alpha-1 collagen type 1 and type I procollagen alpha 1 chain, respectively. These collagens play a vital role in maintaining the integrity and strength of various tissues in the body.
Source	Rabbit
Specificity	The antibody for Collagen I detects the levels of Collagen I that naturally occur in the body.
Species Reactivity	Human;Mouse;Rat
Application	The dilution ratios for WB, IHC, IF/ICC, and ELISA (peptide) are as follows: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000. Please note that the dilution ranges vary for each technique, with WB requiring the most diluted samples, followed by IHC, IF/ICC, and ELISA. WB should be diluted to a ratio of 1:500 to 1:2000, IHC should be diluted to a ratio of 1:50 to 1:200, IF/ICC should be diluted to a ratio of 1:100 to 1:500, and ELISA(peptide) should be diluted to a ratio of 1:20000 to 1:40000. These dilution ratios are crucial for obtaining accurate and reliable results when performing these experiments.
Immunogen	A peptide synthesized from Collagen I found in humans has been created. The new product closely resembles its original source material and has potential applications in various fields where collagen is in demand. Its development is a significant step forward in the field of peptide synthesis, and researchers are hopeful that it will be of great benefit to the medical community. With its use, they anticipate more groundbreaking discoveries and an expanded understanding of the potential applications of peptides in modern medicine.
Properties
Concentration	1mg/ml
Purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography for purification. It was a carefully performed process that guaranteed the highest possible quality of the purified antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	The recommended storage condition for this product is -20 °C for a period of one year. It is important to prevent repeated freeze/thaw cycles.
Storage buffer	The Rabbit IgG solution comes in phosphate buffered saline with a pH of 7.4, 150mM of NaCl, and 0.02% of sodium azide. It is also added with 50% glycerol for stability and should be stored at -20°C. This solution can last for up to 12 months from the date of receipt.
Target
Background	Type I collagen is a member of group I collagen (fibrillar forming collagen).
Tissue specificity	Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Posttranslational modification	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;
UniPort	P08123