Rabbit Anti-IGFBP3 Polyclonal Antibody #abs122920

abs122920-50ul

In Stock

201

abs122920-100ul

In Stock

301

Other names

IGF-BP3, also known as Insulin Like Growth Factor Binding Protein 3, is a crucial component of the 140 K IGF complex. It serves as an acid stable subunit that binds tightly to the complex. This protein is also referred to as Binding protein 29, Binding protein 53, or BP 53. Its role is to interact with growth hormone and modulate its effects by serving as a growth hormone dependent binding protein.
IGF-BP3, alternatively called IBP3, plays a significant role in regulating the activity of insulin-like growth factors (IGFs). It is an essential mediator of their actions within the body. It binds to IGF-1 and IGF-2, thereby increasing their half-life and bioavailability. This interaction helps to prolong the action of IGFs on target cells and tissues.
Furthermore, IGFBP-3 is involved in the regulation of cell growth, differentiation, and apoptosis processes. It exerts its effects through both IGF-dependent and IGF-independent mechanisms. By sequestering IGFs in the circulation and extracellular matrix, it limits their free availability and prevents uncontrolled cellular proliferation.
In summary, IGF-BP3 is a critical component of the IGF system, responsible for modulating the actions of IGF-1 and IGF-2. Its binding to IGFs regulates their bioavailability and influences cell growth and differentiation. The acid-stable subunit of the 140 K IGF complex serves as an important mediator of growth hormone-dependent effects.

Source

Rabbit

Specificity

IGFBP3

Species Reactivity

Human;Mouse;Rat;

Application

The recommended dilutions for WB, ELISA, IHC-P, IHC-F, and IF are as follows: WB 1:500-2000, ELISA 1:500-1000, IHC-P 1:400-800, IHC-F 1:400-800, and IF 1:100-500 (paraffin section requires antigen retrieval). It is important to note that when using paraffin sections for IF, the antigen retrieval process is necessary to ensure accurate results. These dilutions have been determined based on extensive testing and experimentation, and should be followed to achieve optimal results. Additionally, it is recommended to perform proper controls and take other necessary precautions during these procedures to ensure accuracy and reproducibility.

Immunogen

The synthetic peptide KLH-conjugated, derived from the human IGFBP3:186-292/292, can be effectively used in various biological applications. By rearranging the provided information, a highly similar content can be generated. However, let's not rely on the conversation-driven style of text generation but rather focus on creating a completely different speech pattern based on the language model.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To preserve the quality of this item, it is recommended to keep it in a freezer with a temperature of minus 20 degrees Celsius for up to one year. It is important to avoid subjecting it to frequent freezing and thawing cycles, as this may cause damage and lead to loss of potency. Be sure to adhere to these guidelines to ensure the longevity of this product.

Storage buffer

A solution of 0.01M Tris-buffered saline at pH 7.4 has been prepared with the addition of 1% BSA, 0.03% Proclin300, and 50% glycerol. This solution is ideal for use in various biochemical applications due to its stable composition and compatibility with a wide range of reagents and proteins. The addition of glycerol helps to protect the proteins from denaturation and allows for long-term storage at low temperatures. Additionally, the inclusion of Proclin300 helps to prevent microbial growth, ensuring the stability and safety of the solution. Overall, this solution provides a reliable and effective option for biochemical experimentation.

Research area

Growth Factors and Hormones play a crucial role in the development of diabetes through their interaction with Tumor Cell Biology, Immunology, and Transcription Regulators. In the field of Tumor Cell Biology, researchers have discovered that certain growth factors and hormones can promote the growth and proliferation of cancer cells. This interaction has led to the development of potential therapeutic targets for cancer treatment.
Immunology, on the other hand, focuses on the body's immune response to diseases such as cancer and diabetes. Recent studies have shown that growth factors and hormones can modulate immune cell function and influence the progression of these diseases. Understanding these interactions is vital for the development of new immunotherapy strategies for cancer and diabetes.
Transcription regulators are proteins that play a critical role in regulating gene expression. They control the activation or suppression of genes involved in various cellular processes, including tumor cell growth and immunity. Research has revealed that growth factors and hormones can modulate the activity of transcription regulators, resulting in changes in gene expression patterns associated with cancer progression and diabetes.
The relationship between growth factors, hormones, and diabetes is multifaceted. Certain growth factors and hormones, such as insulin and insulin-like growth factor 1 (IGF-1), are essential for glucose homeostasis and the development of diabetes. Dysregulation of these factors can lead to insulin resistance and impaired glucose metabolism, contributing to the onset and progression of diabetes.
In conclusion, the intricate interplay between Tumor Cell Biology, Immunology, Growth Factors and Hormones, Transcription Regulators, and Diabetes underscores the importance of studying these fields together. Unraveling the complex molecular mechanisms underlying these interactions will pave the way for the development of novel therapies and intervention strategies for cancer and diabetes.

Target

Background

GFBP3, a member of the IGFBP family, encodes a protein that contains an IGFBP domain and a thyroglobulin type-I domain. This protein forms a ternary complex with IGFALS and either IGF I or II. By doing so, it circulates in the plasma and extends the half-life of IGFs. Additionally, it modifies the interaction between IGFs and cell surface receptors. Various alternate transcriptional splice variants have been identified, resulting in different isoforms of the protein.

Celluar localization

Secreted protein

UniPort

P17936