Bad Antibody (Center) #abs101775

abs101775-50ul

In Stock

228

Description

This Rabbit Polyclonal Antibody (Pab) has been purified using Peptide Affinity. To create a content highly similar to this, you can rephrase the information. The Pab created using Peptide Affinity is a highly specific antibody that can target a particular antigen. It is produced by immunizing rabbits with the target peptide antigen so that the antibodies produced will have a high affinity for the antigen. The purification process ensures that the final product is of high quality and purity, with fewer non-specific interactions. The resulting Pab is suitable for a wide range of applications such as Western Blotting, Immunohistochemistry (IHC), or ELISA.

Other names

Bcl2-associated agonist of cell death, also known as BAD, is a crucial component that binds to Bcl-2 proteins. Additionally, BAD is often referred to as Bcl-2-like protein 8 or Bcl2-L-8. It is involved in promoting cell death by interacting with Bcl-xL and acting as a death promoter. Moreover, BAD is recognized as a Bcl2 antagonist of cell death, playing a crucial role in regulating cell survival and apoptosis. In summary, BAD, also known as BBC6 or BCL2L8, is an essential protein involved in regulating cell death and maintaining cellular balance.

Source

Rabbit

Specificity

The Bad antibody was produced utilizing KLH conjugated synthetic peptide comprising 53-81 amino acids from the Central region of human Bad, after rabbits were immunized. To craft comparable content, I will rearrange the provided information while adhering to the original text.

Species Reactivity

Human

Application

WB~~1:1000
IHC-P~~1:10~50

Properties

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended to keep the product refrigerated between 2-8°C for up to 2 weeks. For longer periods, it is best to store the product at a temperature of -20°C in small aliquots to prevent any degradation caused by repeated freeze-thaw cycles. It is essential to maintain proper storage conditions to preserve the product's quality and efficacy. Follow these guidelines to ensure maximum retention of the product's qualities.

Storage buffer

This antibody is supplied as a highly purified polyclonal antibody in PBS containing 0.09% (W/V) sodium azide. It is purified using a protein A column, followed by peptide affinity purification.

Research area

As a biologist, I have a great interest in understanding the complexities of diseases like cancer and Amyotrophic Lateral Sclerosis (ALS). These diseases involve cell biology, metabolism, and signal transduction, which are all interconnected processes in the human body. Cancer is a disease caused by the uncontrolled growth of cells, while ALS is a neurodegenerative disease that affects nerve cells in the spinal cord and brain. By studying these diseases, we can gain insight into the underlying mechanisms that contribute to their development and progression. One such mechanism is the abnormal activation of signaling pathways that regulate cell growth and survival. Through continued research, we hope to discover new treatments and therapies that can improve the lives of those affected by these diseases.

Target

Background

By successfully competing for binding to Bcl-X(L), Bcl-2, and Bcl-W, this molecule promotes cell death and disrupts the formation of heterodimers between these proteins and BAX. It specifically counters the death suppressor function of Bcl-X(L) but not that of Bcl-2 (as per similarity). Its role seems to bridge the gap between signaling from growth factor receptors and the activation of apoptotic pathways.

Celluar localization

The cytoplasm is where the phosphorylated protein can be found after it relocates from the outer membrane of the mitochondrion. It's important to note that the original text specifically mentions the outer membrane and the cytoplasm, and this information should be retained in any rephrased content. Therefore, the focus should still be on the movement of the protein from one location to another within the cell.

UniPort

Q92934

References

References

Lee, J.W., et al., Carcinogenesis 25(8):1371-1376 (2004).
Zhang, B., et al., Mol. Cell. Biol. 24(14):6205-6214 (2004).
Ong, C.S., et al., Oncol. Rep. 11(3):727-733 (2004).
Yan, B., et al., J. Biol. Chem. 278(46):45358-45367 (2003).
Taghiyev, A.F., et al., Mol. Cancer Res. 1(7):500-507 (2003).