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Rabbit Anti-AXIN1 Polyclonal Antibody#abs138784

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Description

Catalog-specification	Delivery time	USD price
abs138784-100ug	1-2 Weeks	301.0
abs138784-50ug	1-2 Weeks	201.0

Overview
catalog	abs138784
Other names	Axin 1, also known as Axin-1 or Axis inhibition protein 1, is a protein that plays a critical role in various cellular processes. It is encoded by the AXIN1 gene and is conserved across different species, including mice. Axin 1 is involved in regulating the Wnt signaling pathway, which is essential for embryonic development and tissue homeostasis. The protein Axin 1 acts as a scaffold protein, facilitating the assembly of a protein complex that controls the activity of β-catenin, a key player in the Wnt signaling pathway. This complex also includes other proteins such as GSK-3β, APC, and β-catenin itself. Axin 1 helps in the phosphorylation of β-catenin by promoting its interaction with GSK-3β. This phosphorylation tags β-catenin for degradation, preventing its accumulation and activation of Wnt target genes. In addition to its role in the Wnt pathway, Axin 1 has been implicated in other cellular processes. It interacts with various proteins involved in cell adhesion, cell migration, and cell cycle regulation. Axin 1 is known to interact with proteins such as Cdc42, Disheveled, and JNK, enabling it to influence cytoskeletal dynamics and cell polarity. Mutations in the AXIN1 gene have been linked to several human diseases, including colorectal cancer and hepatocellular carcinoma. These mutations often disrupt the regulation of the Wnt signaling pathway, leading to the aberrant activation of target genes and uncontrolled cell proliferation. In summary, Axin 1 is a crucial protein involved in the regulation of the Wnt signaling pathway and other cellular processes. Its scaffolding function and interactions with various proteins make it a central player in the control of cell behavior and cellular homeostasis. Understanding the mechanisms of Axin 1 function can provide insights into the development of therapeutic approaches for diseases associated with dysregulated Wnt signaling.
Source	Rabbit
Specificity	The AXIN1 Antibody is capable of detecting the total AXIN1 protein at its natural levels in the body. By targeting endogenous levels, this antibody enables researchers to accurately study the presence and activity of AXIN1.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Pig;Dog;Horse;Bovine;
Antigen	AXIN1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human AXIN1.
MW	96 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to perform peptide affinity chromatography in order to purify the antiserum. A highly similar content can be created by rephrasing the original statement as follows: The purification of the antiserum was achieved through the use of peptide affinity chromatography, which involved employing SulfoLink™ Coupling Resin.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at -20 °C for a period of one year. It is important to avoid repeated cycles of freezing and thawing, as this can compromise the effectiveness of the product. Taking necessary precautions while storing the product can help ensure that it remains effective for longer periods of time.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4 and 150mM of NaCl. It also contains 0.02% sodium azide and 50% glycerol to aid in preservation. It is important to store this product at -20 °C and it is considered stable for 12 months from the date of receipt.
Target
Background	Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
Tissue specificity	Ubiquitously expressed.
Posttranslational modification	Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	O15169