Rabbit Anti-MAN1B1 Polyclonal Antibody #abs130315

abs130315-50ug

1-2 Weeks

201

abs130315-100ug

1-2 Weeks

301

Description

The MAN1B1 protein plays a critical role in maintaining the quality of glycoproteins within the cell. Its main function is to identify misfolded glycoproteins and target them for degradation. Specifically, it acts by removing a single alpha-1,2-linked mannose residue from the Man(9)GlcNAc(2) structure, producing Man(8)GlcNAc(2). This process occurs primarily in the ER quality control compartment, where high enzyme concentrations are found. By performing its important trimming function, MAN1B1 helps to ensure that only properly folded glycoproteins are allowed to function within the cell.

Other names

Alpha 1 2 mannosidase is also known as Endoplasmic reticulum alpha mannosidase 1 or Endoplasmic reticulum mannosyl oligosaccharide 1 2 alpha mannosidase 1. It is an enzyme found in the endoplasmic reticulum that plays a crucial role in processing and modifying glycosylated proteins. This enzyme specifically recognizes and cleaves the alpha 1,2-linked mannose residues from Man9GlcNAc2, which is a common glycan structure found in glycoproteins. The proper functioning of this enzyme is essential for ensuring proper protein folding, quality control, and trafficking within the cell. Defects in this enzyme can lead to various glycosylation disorders and contribute to the pathogenesis of certain genetic diseases. Hence, understanding the structure and function of ER alpha 1 2 mannosidase is important for elucidating the intricate processes involved in protein glycosylation and its implications in human health and disease.

Source

Rabbit

Specificity

The detection of endogenous levels of total MAN1B1 is possible using the MAN1B1 antibody. One can generate highly similar content by rearranging the original information while ensuring its accuracy.

Species Reactivity

Human;Mouse;Rat

Application

For Western Blotting, the recommended dilution would be between 1:500 to 1:3000, whereas for IF/ICC the recommended dilution lies in the range of 1:100 to 1:500. For conducting ELISA(peptide), the recommended dilution would be ranging from 1:20000 to 1:40000. It is important to note that these dilutions are optimized for achieving accurate results and ensuring reliable data.

Immunogen

A synthesized peptide derived from human MAN1B1

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is advised to store it at a temperature of -20°C for a duration of one year. It is essential to refrain from exposing it to repeated freeze-thaw cycles as it can cause damage to the product.

Storage buffer

The storage condition for Rabbit IgG is -20 °C in phosphate buffered saline with a pH of 7.4. It is supplemented with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability of the IgG is guaranteed for a period of 12 months starting from the date of receipt. It is important to note that the content generated by rearranging the provided information should be different from the ChapGPT generated content, as per the given instructions.

Target

Background

This protein plays a crucial role in ensuring the quality of glycoproteins by targeting misfolded ones for degradation. Through its actions, it trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to generate Man8GlcNAc2. However, under conditions of high enzyme concentration, as seen in the ER quality control compartment (ERQC), it can trim the carbohydrates even further down to Man5-6GlcNAc2. This process is vital for maintaining proper protein folding and preventing the accumulation of misfolded proteins, which can lead to disease.

Tissue specificity

Widely expressed.

Celluar localization

The Golgi apparatus is responsible for modifying and packaging proteins synthesized in the endoplasmic reticulum, before they are transported to the extracellular region or secreted.

UniPort

Q9UKM7