P16INK4a Antibody (C-term E119) #abs101168

abs101168-50ul

In Stock

228

Description

Affinity purified rabbit polyclonal antibody (Pab) is a peptide-specific antibody that has undergone purification using affinity chromatography techniques. This antibody is generated from rabbits immunized with a specific peptide of interest. The affinity purification process allows for the isolation of antibodies that specifically bind to the target peptide, resulting in a highly specific and purified antibody preparation. The use of rabbit as the host organism provides advantages such as high antibody yield and excellent epitope recognition. This Pab can be a valuable tool in various research applications, including immunohistochemistry, western blotting, and ELISA assays, allowing for the detection and quantification of the target peptide in samples. Overall, affinity purified rabbit polyclonal antibodies offer researchers a reliable and effective tool for studying specific peptides and their associated functions.

Other names

The gene known as Cyclin-dependent kinase inhibitor 2A has three isoforms: isoform 1, isoform 2, and isoform 3. It is also referred to as Cyclin-dependent kinase 4 inhibitor A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A, CDKN2A, CDKN2, and MTS1. This gene is essential for cell cycle regulation as it encodes for a protein that inhibits the activity of cyclin-dependent kinases such as CDK4. CDK4I, or p16INK4A, is a well-known tumor suppressor that prevents the uncontrolled growth of cells and the development of cancer. Its loss or inactivation is frequently observed in various types of tumors, making it an attractive target for cancer therapies.

Source

Rabbit

Specificity

The p16INK4a antibody has been produced by immunizing rabbits with a synthetic peptide that is conjugated to KLH. This peptide corresponds to amino acids 104-131 from the C-terminal region of the human p16INK4a protein. I will now generate a highly similar content by rearranging the information provided, while ensuring that the meaning remains intact.
The p16INK4a antibody is derived from rabbits that were immunized with a synthetic peptide comprising amino acids 104-131 from the C-terminal region of the human p16INK4a protein. The peptide was conjugated to KLH to enhance the immune response in the rabbits.

Species Reactivity

Human

Application

FC~~1:25
WB~~1:2000

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

To ensure optimal preservation, it is recommended to keep the refrigerated item within the temperature range of 2-8°C for a maximum duration of 2 weeks. For extended storage, it is advised to store the item at -20°C in small portions to prevent any potential damage caused by freeze-thaw cycles.

Storage buffer

This polyclonal antibody has been purified using a protein A column and peptide affinity purification, and is supplied in PBS containing 0.09% (W/V) sodium azide. The resulting product is a highly purified antibody that is ready to use for a variety of research applications. The peptide affinity purification step ensures high specificity and sensitivity, making this antibody an ideal tool for detecting your target of interest. The inclusion of sodium azide helps to prevent bacterial growth and ensure the stability of the antibody during storage. Whether you are using this antibody for Western blotting, immunofluorescence, or any other application, you can be confident in its purity and reliability.

Research area

Cancer;Cell Biology

Target

Background

By strongly interacting with CDK4 and CDK6, this regulatory protein serves as a powerful inhibitor of normal cell proliferation. It effectively prevents the interaction between CDK4/6 and cyclins D, hindering their ability to phosphorylate the retinoblastoma protein.

Celluar localization

Cytoplasm. Nucleus

UniPort

P42771

References

References

Kovacs, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(12):5429-5434(2010)
Irvine, M., et al. Cell Cycle 9(4):829-839(2010)
Zhang, H.J., et al. J. Cell. Biochem. 106(3):464-472(2009)
Ivanchuk, S.M., et al. Cell Cycle 7(12):1836-1850(2008)
Bandyopadhyay, K., et al. Biochemistry 46(49):14325-14334(2007)