Phospho-FANCG(Ser383) Rabbit Polyclonal Antibody #abs130022

abs130022-50ug

1-2 Weeks

201

abs130022-100ug

1-2 Weeks

301

Description

FANCG is a crucial DNA repair protein that likely functions in either postreplication repair or as a cell cycle checkpoint regulator. It has been implicated in maintaining normal chromosome stability and repairing interstrand DNA cross-links, making it a potential tumor suppressor gene. FANCG is a part of the multisubunit FA complex, which includes FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, and FANCM. Notably, this complex is not found in individuals with FA.

Other names

The XRCC9 protein, also known as FANCG or Fanconi anemia complementation group G, is a key player in DNA repair. It is involved in the repair of damaged DNA and is essential for maintaining the stability of the genome. XRCC9 has also been linked to Fanconi anemia, a rare genetic disorder that affects the body's ability to repair DNA damage. The protein is sometimes referred to as protein FACG or X-ray repair, complementing defective, in Chinese hamster cells 9. It plays a crucial role in repairing DNA damage caused by exposure to radiation or chemicals, and mutations in XRCC9 can lead to a range of health issues. Despite being an important player in DNA repair, the exact mechanisms by which XRCC9 works are still being explored by researchers.

Source

Rabbit

Specificity

The Phospho-FANCG(Ser383) Antibody is designed to specifically target and detect levels of FANCG that have been phosphorylated at Sersine 383. This means that it is capable of detecting the presence of endogenous levels of FANCG, but only when it is phosphorylated at this specific site. Whether there are other sites where FANCG can be phosphorylated remains uncertain, but this antibody is designed to recognize Sersine 383 specifically. Therefore, it serves as a useful tool for researchers who are interested in studying FANCG and its functions within the cell. By detecting phosphorylation at this specific site, this antibody provides valuable insights into the role of FANCG in cellular processes such as DNA repair and maintenance.

Species Reactivity

Human

Application

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Immunogen

A peptide has been synthesized from the human FANCG protein, specifically targeting the serine 383 phosphorylation site. The resulting peptide closely mirrors the original protein sequence and is intended for use in further studies on FANCG function and regulation. This peptide may prove useful in elucidating the precise mechanisms by which FANCG is phosphorylated and how this modification affects its activity. By analyzing the properties of this synthesized peptide, researchers may gain new insights into the complex biology of FANCG and its role in human health and disease.

Properties

Concentration

1mg/ml

Purification

The purified rabbit serum was utilized to obtain the antibody through a process of sequential chromatography on phospho- and non-phospho-peptide affinity columns followed by affinity purification. A highly similar version of this information can be generated by rephrasing it as "Through a rigorous process of affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns, the antibody was successfully extracted from purified rabbit serum."

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of your product, it is important to store it at -20 °C for up to one year. It is best to avoid any repeated freeze/thaw cycles to maintain the integrity of the item. Keep in mind these guidelines to ensure the quality of your product.

Storage buffer

This product contains Rabbit IgG in a phosphate buffered saline solution with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, making it suitable for long-term storage at -20 °C. It is guaranteed to remain stable for up to 12 months from the date of receipt, ensuring that you can rely on its efficacy over an extended period of time.

Target

Background

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.

Tissue specificity

Highly expressed in testis and thymus. Found in lymphoblasts.

Celluar localization

Cytosol;Mitochondrion;Nucleus;Plasma Membrane;

UniPort

O15287