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SMPD1 Polyclonal Antibody #abs117402

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent. Data Examples To analyze the levels of SMPD1 protein in various cell lines, Western blot was performed using the SMPD1 antibody (abs117402) at a...

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Description

Catalog-specification	Delivery time	USD price
abs117402-50ul	In Stock	228

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
Other names	ASM;ASMASE;NPD
Source	Rabbit
Species Reactivity	Human;Mouse;Rat
Application	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Immunogen	The recombinant fusion protein comprises a fragment derived from human SMPD1 (NP_000534.3), specifically encompassing amino acids 70 to 340. We aim to create an analogous content by employing the provided information but presenting it in a distinct manner.
Properties	Mouse kidney,Mouse heart,Mouse lung,Rat liver
Properties
Purification	Affinity purification
Clonality	Polyclonal Antibody
Isotype	IgG
Stability & Storage	Store at -20℃. Avoid freeze / thaw cycles.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Here's a rephrased version of the original content: To create a buffer solution for PBS, add 0.02% sodium azide and 50% glycerol to achieve a pH level of 7.3. This should be mixed well and stored in a solution containing PBS. The addition of sodium azide will help prevent bacterial growth and ensure the solution remains stable. Glycerol is added to improve the overall viscosity of the solution and protect it from freezing or boiling. Overall, this buffering solution is an important component in scientific research and necessary for experiments involving PBS.
Target
Background	This gene encodes a crucial protein that plays a significant role in lysosomal function by converting sphingomyelin into ceramide. Moreover, this protein demonstrates phospholipase C activity. However, genetic mutations in this gene may lead to two forms of Niemann-Pick disease: type A and type B. Interestingly, several transcript variants of this gene have been observed, all of which code for different protein isoforms with distinctive characteristics.
Celluar localization	Lysosome
UniPort	P17405

Data Examples

To analyze the levels of SMPD1 protein in various cell lines, Western blot was performed using the SMPD1 antibody (abs117402) at a dilution of 1:1000. The secondary antibody used was the HRP Goat Anti-Rabbit IgG (H+L), which was diluted at 1:10000. For each lane, 25ug of lysates/proteins were used and blocked using 3% nonfat dry milk in TBST. The detection method used was the ECL Basic Kit, and the exposure time was set to 90 seconds. This procedure allowed for the detection and quantification of SMPD1 protein levels in the different cell lines.