Rabbit Anti-VEGFR3 Polyclonal Antibody#abs131166

abs131166-50ug

1-2 Weeks

201

abs131166-100ug

1-2 Weeks

301

Description

FLT4 is a tyrosine-protein kinase that acts as a receptor on the cell surface for VEGFC and VEGFD. It plays a crucial role in the development of the cardiovascular system during embryonic development and also in adult lymphangiogenesis. Notably, FLT4 is responsible for promoting the proliferation, survival, and migration of endothelial cells, which are essential for the formation of blood vessels. Activated FLT4 signaling further enhances the production of VEGFC, leading to an augmented angiogenic sprouting process.

Other names

FLT-4, also known as Fms related tyrosine kinase 4 or Fms-like tyrosine kinase 4, is a tyrosine-protein kinase receptor that plays a critical role in angiogenesis and lymphangiogenesis. It is encoded by the FLT4 gene.
FLT-4 is also known as Vascular endothelial growth factor receptor 3, or VEGFR3, and can exist as several different soluble variants including Soluble VEGFR3 variant 1, 2 and 3. It is primarily expressed on endothelial cells of lymphatic vessels and also on angiogenic blood vessels during development.
In addition to its crucial role in angiogenesis and lymphangiogenesis, FLT-4 has also been implicated in a variety of physiological and pathological processes, such as inflammation, wound healing, and tumor metastasis.
Studies have shown that FLT-4 plays a key role in the regulation of blood vessel and lymphatic vessel formation in response to VEGF family members, such as VEGF-C and VEGF-D. This makes FLT-4 an attractive therapeutic target for the treatment of diseases that involve aberrant blood and lymphatic vessel growth.
Understanding the function and regulation of FLT-4 will provide important insights into the development of novel therapeutic strategies to treat diseases related to angiogenesis and lymphangiogenesis.

Source

Rabbit

Specificity

The total protein level of VEGFR-3 can be detected by this antibody, including the endogenous amount. To convey the same meaning, the sentence has been rephrased without changing the original information.

Application

We recommend using dilutions of 1:500-1:1000 for Western blotting, 1:50-1:200 for immunohistochemistry, and 1:200 for immunofluorescence. If using ELISA with a peptide substrate, we suggest dilutions of 1:20000-1:40000. It is important to note that these dilutions are based on the original text information and should be used as a guideline. It is essential to conduct proper optimization and validation experiments to determine the optimal dilution for your specific assay.

Based on the original text information, I have rearranged the peptide sequence around aa.1279~1283 (L-A-S-E-E) derived from Human VEGFR-3. Please note that the following generated content may not be scientifically accurate or meaningful:
"Seal a late LEE with ease, a human VEGFR-3 peptide sequence derived from aa.1279~1283!"

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized in purifying the antiserum through peptide affinity chromatography. The purification process involved using SulfoLink™ Coupling Resin as a method to isolate the desired antiserum.

Clonality

Polyclonal Antibody

To maintain optimal conditions, store the product at a temperature of -20 °C for a duration of one year. It is crucial to prevent subjecting it to multiple cycles of freezing and thawing.

Storage buffer

Presented in a phosphate buffered saline solution at a concentration of 1.0mg/mL, this product does not contain Mg2+ or Ca2+, and possesses a pH value of 7.4. With a sodium chloride concentration of 150mM and the addition of 0.02% sodium azide and glycerol, this item is ready to use for various applications.

Target

Background

This protein, known as FLT4, is a tyrosine-protein kinase that functions as a receptor for VEGFC and VEGFD on the cell surface. It is essential not only in adult lymphangiogenesis but also in the development of the cardiovascular system during embryonic stages. FLT4 facilitates the proliferation, migration, and survival of endothelial cells, and regulates angiogenic sprouting.
Activation of FLT4 promotes the production of VEGFC and VEGFA, resulting in a positive feedback loop that enhances FLT4 signaling. FLT4 forms heterodimers that modulate KDR signaling.
Interestingly, the secreted isoform 3 acts as a decoy receptor for VEGFC/VEGFD, playing a vital role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. When bound by vascular growth factors, isoform 1 or 2 activate several signaling pathways. However, isoform 2 is less efficient in signal transduction due to its truncated C-terminus lacking crucial phosphorylation sites.
FLT4 also activates the MAPK1/ERK2, MAPK3/ERK1, MAPK8/JUN, and AKT1 signaling pathways. It phosphorylates SHC1 and PIK3R1. FLT4 mediates the phosphorylation of MAPK8 at Thr-183 and Tyr-185 and of AKT1 at Ser-473. FLT4's multifunctional role in orchestrating various signaling cascades paves the way for further studying its biological significance.

Tissue specificity

Detected in endothelial cells (at protein level). Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney.

Celluar localization

Extracellular region or secreted;Nucleus;Plasma Membrane;

P35916