Rabbit Anti-IkappaB-alpha Polyclonal Antibody#abs131168

abs131168-50ug

1-2 Weeks

201

abs131168-100ug

1-2 Weeks

301

Description

The NFKB complex, which plays a crucial role in immune response and inflammation, is formed by the binding of NFKB1 or NFKB2 with REL, RELA or RELB. In order to maintain tight regulation of this complex, I-kappa-B proteins act as inhibitors, sequestering it within the cytoplasm and preventing its activation. This intricate control is essential for preventing the dysregulation of NF-kappa-B signaling, which has been implicated in a range of diseases, from cancer to chronic inflammation. By understanding the complex interplay between these molecules, we can gain insights into the underlying mechanisms of these disorders and develop targeted therapeutic interventions to improve patient outcomes.

Other names

The protein I kappa B alpha is also known as IKBA or NF-kappa-B inhibitor alpha. It plays a critical role in regulating the activity of the protein complex NF-kappa-B, which is involved in multiple cellular processes such as inflammation, immune response, and cell survival. I kappa B alpha binds to NF-kappa-B, preventing its translocation into the nucleus where it can activate target genes. The gene encoding I kappa B alpha is called NFKBIA and is located on chromosome 14 in humans. Mutations in NFKBIA have been associated with various diseases including cancer, autoimmune disorders, and inflammatory diseases. The full name of I kappa B alpha is nuclear factor of kappa light chain gene enhancer in B cells inhibitor alpha, and it is also known as major histocompatibility complex enhancer-binding protein MAD3 or MAD3.

Source

Rabbit

Specificity

The total levels of endogenous IkappaB-alpha can be accurately identified using the IkappaB-alpha Antibody.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine

Application

The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. It is important to adhere to these dilution ranges to achieve optimal results in your experiments.

Human IkappaB-alpha is the source from which a synthesized peptide is derived. To create a similar content, one can rearrange the original information by ensuring that the message remains based on the text.

Properties

1mg/ml

Purification

Through the utilization of SulfoLink™ Coupling Resin, peptide affinity chromatography was employed in order to purify the antiserum.

Clonality

Polyclonal Antibody

-20°C。/。

Storage buffer

Please note that the generated content is for informational purposes only and should not be considered accurate or reliable.
The storage conditions for Rabbit IgG are as follows: it should be stored at a temperature of -20 °C. The antibody is stable for a period of 12 months starting from the date of receipt.
The Rabbit IgG is formulated in phosphate buffered saline with a pH value of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol.
It is important to maintain the pH value at 7.4 and the concentration of NaCl in order to preserve the stability and functionality of the antibody. The addition of sodium azide helps to prevent microbial contamination. Glycerol is included in the formulation as a cryoprotectant, aiding in the long-term storage at sub-zero temperatures.
Please ensure that the storage temperature is maintained consistently to ensure the integrity and efficacy of the Rabbit IgG. Always reference the date of receipt to determine its stability and usability.

Target

Background

The cytoplasmic localization signals of REL dimers are masked by this protein, leading to the inhibition of dimeric NF-kappa-B/REL complexes' activity. Upon cellular stimulation caused by immune and proinflammatory responses, phosphorylation occurs, promoting degradation through ubiquitination. Consequently, the dimeric RELA is able to translocate to the nucleus and activate transcription.

Tissue specificity

Induced in adherent monocytes.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P25963