Rabbit Anti-MMP3 Polyclonal Antibody #abs130153

abs130153-50ug

In Stock

201

abs130153-100ug

In Stock

301

Description

MMP3, also known as matrix metalloproteinase 3, is a proteolytic enzyme that can degrade various extracellular matrix components, including fibronectin, laminin, gelatins (type I, III, IV, and V), collagens (III, IV, X, and IX), and cartilage proteoglycans. It belongs to the peptidase M10A family and is capable of activating procollagenase. This information has been sourced from UniProtKB. Please note that the generated content has been rearranged to provide a similar description while maintaining the original text information.

Other names

Matrix metalloproteinase 3, also known as MMP-3 or stromelysin-1, is an enzyme that plays an important role in tissue remodeling and repair. It is encoded by the Mmp3 gene and is expressed in a variety of cells, including fibroblasts, chondrocytes, and macrophages.
MMP-3 is a proteoglycanase, which means that it is capable of breaking down proteoglycans – complex molecules that provide structural support and cushioning in connective tissues such as cartilage. This makes MMP-3 a key player in diseases such as osteoarthritis, where the breakdown of cartilage is a major contributor to joint damage.
In addition to its role in tissue remodeling and repair, MMP-3 is also involved in inflammation. It is known to cleave and activate pro-inflammatory cytokines such as IL-1β, as well as degrade various extracellular matrix proteins.
MMP-3 has been implicated in a number of pathological conditions, including cancer and cardiovascular disease. It is therefore a potential target for therapeutic intervention in these diseases.
Overall, MMP-3 is a versatile and important enzyme that plays a critical role in regulating tissue homeostasis and response to injury.

Source

Rabbit

Specificity

The detection of total MMP3 at endogenous levels can be accomplished using the MMP3 antibody.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Horse

Application

The dilutions for Western blot (WB) range from 1:500 to 1:3000, for immunohistochemistry (IHC) from 1:50 to 1:200, for immunofluorescence/immunocytochemistry (IF/ICC) from 1:100 to 1:500, and for ELISA (peptide) from 1:20000 to 1:40000. These dilution ranges represent the recommended concentrations for each respective technique and are crucial for obtaining accurate and reliable results in protein detection and analysis. It is essential to carefully follow these dilution guidelines to ensure optimal performance and sensitivity in the experiments conducted.

Immunogen

A synthesized peptide derived from human MMP3.

Properties

Concentration

1mg/ml

Purification

To obtain a purified antiserum, we utilized SulfoLink™ Coupling Resin in peptide affinity chromatography. This process effectively isolated and cleansed the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

The recommended storage condition for one year is at -20 °C. It is crucial to avoid subjecting the product to repeated freeze/thaw cycles during this period.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4. The solution also includes 150mM NaCl, 0.02% sodium azide, and 50% glycerol for stabilization. It is recommended to store this product at -20 °C for optimal stability, and it is guaranteed to be stable for at least 12 months from the date of receipt.

Target

Background

This enzyme has the ability to break down several extracellular matrix molecules, including fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Moreover, it is capable of activating procollagenase, which is crucial for the remodeling and tissue repair processes. Overall, this enzyme plays a significant role in maintaining the structural integrity of tissues and facilitating cellular functions such as proliferation, migration, and differentiation.

Celluar localization

Extracellular region or secreted;

UniPort

P08254