Rabbit Anti-eNOS Polyclonal Antibody #abs119744

abs119744-50ul

In Stock

201

abs119744-100ul

In Stock

301

Other names

Endothelial nitric oxide synthase (eNOS), also known as NOS-3, cNOS, or constitutive NOS, is an enzyme responsible for the production of nitric oxide (NO) in endothelial cells. This enzyme plays a crucial role in regulating various physiological processes in the cardiovascular system. Additionally, eNOS is also referred to as EC NOS, ecNOS, or endothelial nitric oxidase synthase. It is encoded by the NOS3 gene and is specifically expressed in endothelial cells.
The function of eNOS is to convert the amino acid L-arginine into L-citrulline and NO. Nitric oxide is a potent signaling molecule that is involved in the regulation of blood pressure, vasodilation, and inhibition of platelet aggregation. It also participates in the maintenance of vascular integrity and acts as a protective factor to prevent the development of atherosclerosis.
The activity of eNOS is primarily regulated by calcium/calmodulin binding and phosphorylation events. Dysfunction or decreased expression of eNOS has been associated with various cardiovascular diseases, including hypertension, coronary artery disease, and endothelial dysfunction.
In summary, endothelial nitric oxide synthase (eNOS) is an essential enzyme involved in the production of nitric oxide, which plays a critical role in maintaining cardiovascular homeostasis and preventing the development of vascular diseases.

Source

Rabbit

Specificity

eNOS

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;Sheep;Guinea Pig;

Application

To use ELISA, a recommended dilution of 1:500-1000 is suggested. For IHC-P, a dilution of 1:400-800 is appropriate, while for IHC-F, a dilution of 1:400-800 is also recommended. IF requires a dilution of 1:100-500, but keep in mind that paraffin section will need antigen retrieval. Remember to follow proper protocols and recommendations when working with these techniques to ensure accurate and reliable results.

Immunogen

The synthetic peptide KLH-conjugated, derived from the human NOS-3 C-terminus at residues 1105 to 1202/1203, demonstrates great similarity to the original text.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

It is recommended to store the item at a temperature of -20 °C for a year. It is important to note that you should avoid any instances of freezing and thawing cycles repeatedly. Ensure that the item is stored in a manner that preserves its integrity and potency over time. Taking these precautions will help maintain the quality of the item for an extended period.

Storage buffer

In a pH7.4 TBS solution, containing 0.01M of the compound, 1% BSA, 0.03% Proclin300, and 50% Glycerol are present. The content is designed to provide optimal conditions for certain chemical reactions and biological assays. The addition of BSA helps to stabilize proteins and prevent degradation, while Proclin300 serves as a preservative to prevent bacterial growth. Finally, the inclusion of glycerol enhances the solubility of hydrophobic molecules and reduces the tendency of proteins to aggregate. Overall, this mixture is a crucial tool in various research and analytical applications.

Research area

Neurobiology

Target

Background

Nitric oxide synthase (NOS) is an enzyme that catalyzes the oxidation of a guanidine nitrogen in arginine, resulting in the release of nitric oxide, a free radical, as well as citrulline. The nitric oxide produced by NOS serves as a messenger in various biological processes, including vasodilation, neurotransmission, and anti-tumor and anti-pathogenic activities. NOS is categorized into three types: neuronal NOS (nNOS) or brain NOS (bNOS), inducible NOS (iNOS) or macrophage NOS (mNOS), and endothelial NOS (eNOS).
eNOS, a calcium/calmodulin dependent enzyme, undergoes several post-translational modifications, such as acylation with myristate and palmitate, as well as phosphorylation on multiple residues. Similar to other members of the NOS family, eNOS produces the diffusible second messenger nitric oxide through a series of reactions involving L-arginine, with L-citrulline being an intermediate. eNOS plays a crucial role in the regulation of vascular tone, platelet aggregation, and cardiac myocyte function.

Celluar localization

Cytoplasm cell membrane

UniPort

P29474