Rabbit Anti-HSP60 Polyclonal Antibody #abs119768

abs119768-50ul

In Stock

201

abs119768-100ul

In Stock

301

Other names

The HSP 60 protein, also known as HSP-60 or 60 kDa chaperonin, is a mitochondrial heat shock protein. It is also referred to as 60kDa, cb863, Chaperonin 60, CPN 60, CPN60, fa04a05, fb22d10, fi27b05, GROEL, GroEL Homolog, GroEL, E. coli, homolog of, Heat shock 60kD protein 1 (chaperonin), Heat shock 60kD protein 1 chaperonin, heat shock 60kDa protein 1 (chaperonin), Heat Shock Protein 60, Heat shock protein 65, HLD4, Hsp 60, HSP 65, HSP65, HSPD 1, HSPD1, HuCHA60, id:ibd2197, Mitochondrial matrix protein P1, P60 lymphocyte protein, sb:cb144, Short heat shock protein 60 Hsp60s1, 60 kDa heat shock protein, mitochondrial, and Chaperonin 60.
This highly conserved protein plays a critical role in protein folding and quality control within the mitochondria. It assists in proper folding of newly synthesized proteins and prevents the aggregation of damaged or misfolded proteins. HSP 60 is involved in cellular stress responses, as it is upregulated under conditions of environmental stress, such as heat shock.
HSP 60 has been extensively studied and is found in various organisms, including bacteria, plants, and animals. In humans, HSP 60 is encoded by the HSPD1 gene. Mutations in this gene are associated with certain neurodegenerative disorders and autoimmune diseases.
In summary, HSP 60 is a vital chaperonin protein involved in protein folding and cellular stress responses. Its diverse names reflect its importance and widespread presence in different organisms.

Source

Rabbit

Specificity

HSP60

Species Reactivity

Human;Mouse;Rat;Dog;Cow;Horse;Rabbit;

Application

The recommended dilution for WB is 1:500-2000, for ELISA it is 1:500-1000, for IHC-P it is 1:400-800, and for IHC-F it is 1:400-800. For IF, the recommended dilution is 1:100-500, with antigen retrieval needed for paraffin sections. To ensure accurate results, it is important to follow these dilution guidelines when using the specified assays.

Immunogen

A synthetic peptide derived from the C-terminus of human HSP60, conjugated with KLH and spanning residues 501-573 out of a total of 573, has been generated for research purposes. Similar peptide sequences may be derived and studied to gain insight into the function and interactions of HSP60 in the cellular environment. Through careful analysis and experimentation, researchers may uncover new avenues for therapeutic interventions for diseases related to HSP60 dysfunction.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To maintain the quality, store for one year at -20 °C and prevent subjecting the product to multiple cycles of freezing and thawing. It is crucial to avoid repeating the process of freezing and thawing to ensure optimal storage conditions.

Storage buffer

0.01M TBS(pH7.4)，1% BSA，0.03% Proclin30050%。

Research area

Signal transduction is a crucial process in tumor immunology that involves the transmission of molecular signals within cells. One aspect of this process is the regulation of gene expression through transcription factors. These proteins bind to specific DNA sequences and can either activate or repress the transcription of target genes. Additionally, mitochondria play a significant role in tumor immunology. They are responsible for producing energy, maintaining cellular homeostasis, and regulating apoptotic signaling pathways. The dysregulation of mitochondrial function can contribute to the development and progression of tumors.

Target

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

Celluar localization

Cytoplasm mitochondria

UniPort

P10809