Rabbit Anti-MMP7 Polyclonal Antibody #abs130154

abs130154-50ug

1-2 Weeks

201

abs130154-100ug

1-2 Weeks

301

Description

MMP7 belongs to the peptidase M10A family and is known to degrade various proteins including casein, gelatins of types I, III, IV, and V, as well as fibronectin. Additionally, this enzyme is capable of activating procollagenase. This information has been sourced from UniProtKB.

Other names

Matrilysin, also known as Matrin or Matrix Metalloproteinase 7 (MMP-7), is an enzyme belonging to the matrix metalloproteinase family. It is encoded by the MMP7 gene in humans. Other names and aliases for this protein include MMP 7, MMP7_HUMAN, MPSL1, PUMP 1, Pump-1 protease, Pump1, Uterine matrilysin, and Uterine metalloproteinase.
Matrilysin is an important protease that plays a crucial role in various physiological and pathological processes. It is involved in tissue remodeling, cell migration, and epithelial cell shedding. Additionally, matrilysin has been implicated in several diseases, including cancer, inflammation, and fibrosis.
This enzyme is known for its ability to degrade extracellular matrix components, such as collagen and laminin. It is mainly expressed in epithelial cells and is particularly abundant in the gastrointestinal tract, respiratory system, and reproductive organs.
The activity of matrilysin is tightly regulated to prevent excessive tissue degradation. It is produced as an inactive zymogen that requires activation in order to become enzymatically active. Various factors, such as other proteases and pro-inflammatory cytokines, can initiate the activation of matrilysin.
Understanding the function and regulation of matrilysin is essential for the development of targeted therapies for diseases involving excessive tissue remodeling. Researchers continue to investigate the precise roles of this enzyme and its potential as a therapeutic target.
In conclusion, matrilysin, also referred to as Matrin or Matrix Metalloproteinase 7 (MMP-7), is a crucial enzyme in the matrix metalloproteinase family. It plays diverse roles in tissue remodeling, cell migration, and proteolysis. Further exploration of its mechanisms of action and potential therapeutic applications is ongoing.

Source

Rabbit

Specificity

The MMP7 antibody is capable of detecting total levels of endogenous MMP7 protein in cells. By using this antibody, researchers can accurately assess the expression levels of MMP7 in various applications. With its high specificity, this antibody ensures that only the target protein is detected, enabling more accurate experimental results.

Species Reactivity

Human;Mouse;Rat

Application

For Western blot, the recommended dilution range is 1:500 to 1:3000, while for Immunohistochemistry, a dilution range of 1:50 to 1:200 is suggested. When using Immunofluorescence or Immunocytochemistry, a dilution range of 1:100 to 1:500 is recommended. For ELISA with peptide, the recommended dilution range is between 1:20000 to 1:40000. These guidelines are based on the original information provided and are intended to help users select the appropriate dilution for their specific experiment.

Immunogen

A synthesized peptide derived from human MMP7.

Properties

Concentration

1mg/ml

Purification

To purify the antiserum, a peptide affinity chromatography technique was utilized with the aid of SulfoLink™ Coupling Resin. This helped to ensure that a highly specific and effective purification process was achieved for the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this may compromise its integrity.

Storage buffer

The storage instructions for Rabbit IgG in phosphate buffered saline are as follows: it should be stored at a temperature of -20 °C. It is important to note that the solution is stable for a period of 12 months from the date of receipt. The composition of the solution includes phosphate buffered saline with a pH value of 7.4, 150mM NaCl, and 0.02% sodium azide. Additionally, the solution contains 50% glycerol. To maintain the stability and efficacy of the product, it is crucial to store it under the recommended conditions.

Target

Background

The enzymatic activity of degrading casein, gelatins of types I, III, IV, and V, and fibronectin is observed. Additionally, it facilitates the activation process of procollagenase.

Celluar localization

Extracellular region or secreted;

UniPort

P09237