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Rabbit Anti-AXL Polyclonal Antibody#abs140241

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Description

Catalog-specification	Delivery time	USD price
abs140241-50ug	1-2 Weeks	201.0
abs140241-100ug	1-2 Weeks	301.0

Overview
catalog	abs140241
Other names	Adhesion Related Kinase (ARK), also known as AXL oncogene or AXL receptor tyrosine kinase, is an important protein that plays a critical role in various cellular functions. It is encoded by the AI323647 gene and belongs to the Tyro7 family of receptor tyrosine kinases. The AXL transforming sequence/gene is responsible for the transformation of normal cells into cancerous ones. Moreover, AXL is an oncogene that has been shown to be overexpressed in many types of cancers. AXL is also known as Tyrosine-Protein Kinase Receptor UFO, and JTK11 or UFO_HUMAN. It is involved in cellular processes, including cell adhesion, migration, invasion, and apoptosis. Additionally, AXL is an important kinase that regulates immune cell function and is involved in the pathogenesis of autoimmune diseases. In summary, AXL is critical for cellular function and is involved in various biological processes such as cancer, immune cell function, and autoimmune diseases.
Source	Rabbit
Specificity	The expression of total AXL can be identified through the use of AXL Antibody, which is capable of detecting endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Antigen	AXL
Application	ELISA (peptide) is performed at a dilution range of 1:20000-1:40000. For immunofluorescence/immunocytochemistry (IF/ICC), the recommended dilution is 1:100-1:500, while for Western blotting (WB), the dilution is suggested to be 1:1000-3000. It is important to ensure that the generated content adheres to the original text information while presenting it in a different manner.
Immunogen	A synthesized peptide derived from human AXL.
MW	97.4kDa
Properties
Concentration	1mg/ml
purification	In order to purify the antiserum, SulfoLink™ Coupling Resin was utilized in the process of peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	It is recommended to store the product at a temperature of -20 °C for a duration of one year to ensure its longevity. It is important to avoid subjecting it to multiple cycles of freezing and thawing, as it may affect its quality.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This mixture is stable for a period of 12 months starting from the date of receipt. It is important to note that the generated content below is a highly similar rearrangement of the original information: To ensure the stability and integrity of Rabbit IgG, it is recommended to store it at a temperature of -20 °C. The storage buffer consists of phosphate buffered saline with a pH of 7.4, along with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This optimized formulation allows for long-term storage without compromising the quality of the product. Please make sure to note the date of receipt, as the Rabbit IgG solution remains stable for a duration of 12 months under proper storage conditions.
Target
Background	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
Tissue specificity	Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Posttranslational modification	Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P30530