Phospho-GABA-RB (Ser434) Rabbit Polyclonal Antibody#abs130834

abs130834-50ug

1-2 Weeks

201

abs130834-100ug

1-2 Weeks

301

Description

The GABA receptor plays a crucial role in the functioning of the vertebrate brain by facilitating neuronal inhibition. This effect is achieved by binding GABA, the major inhibitory neurotransmitter, to the GABA/benzodiazepine receptor, which in turn opens a chloride channel integral to the receptor complex.

Other names

GABA(A) receptor beta 1, also known as AW061132 or B230208N19Rik, is a crucial component of the GABA(A) receptor. It plays a vital role in regulating inhibitory neurotransmission in the brain. The GABA(A) receptor beta 1 subunit is encoded by the GABRB1 gene.
This gene is responsible for encoding the polypeptide of the GABA(A) receptor beta 1 subunit. The receptor is an important target for drugs such as benzodiazepines, which enhance its activity and have sedative and anxiolytic effects. The GABA(A) receptor beta 1 subunit is widely expressed in the central nervous system and is involved in mediating the effects of the neurotransmitter gamma-aminobutyric acid (GABA).
The GABA(A) receptor is a pentameric complex composed of five subunits, including two alpha, two beta, and one gamma subunit. The GABA(A) receptor beta 1 subunit is a crucial component for the assembly and functional properties of the receptor.
Research studies have demonstrated that mutations in the GABRB1 gene can lead to various neurological disorders, including epilepsy and autism spectrum disorders. These mutations disrupt the normal function of the GABA(A) receptor, resulting in altered neuronal excitability and neurotransmission.
In summary, GABA(A) receptor beta 1, encoded by the GABRB1 gene, is a fundamental component of the GABA(A) receptor. It is involved in regulating inhibitory neurotransmission and has implications in various neurological disorders. Understanding the role of GABA(A) receptor beta 1 in the brain is critical for developing potential therapeutic strategies targeting this receptor.

Source

Rabbit

Specificity

The Phospho-GABA-RB (Ser434) Antibody is designed to detect the presence of endogenous levels of GABA-RB, but only when it is phosphorylated at Serine 434. This antibody works by recognizing the specific phospho-epitope and distinguishing it from the unphosphorylated GABA-RB protein. This helps researchers to study the role of phosphorylation in GABA-RB function and regulation. Through the use of this highly specific antibody, researchers can gain valuable insights into the molecular mechanisms that underlie GABA-RB signaling and related processes.

Predictive reaction species

Rabbit;Pig;Dog;Horse;Bovine

Application

For western blotting applications, dilute the antibody at a range of 1:500-1:2000. When using the antibody for immunohistochemistry, dilute it at a range of 1:50-1:200. For immunofluorescence and immunocytochemistry applications, dilute the antibody at a range of 1:100-1:500. If using the antibody in peptide ELISA, dilute at a range of 1:20000-1:40000. It is essential to maintain the specified dilution range for successful and accurate results.

The phosphorylation site of Serine 434 has been utilized to synthesize a peptide derived from human GABA-RB. It is important to note that the content generated should be based on the original text information but presented in a highly similar manner.

Properties

1mg/ml

Purification

Sequential chromatography on phospho- and non-phospho-peptide affinity columns was utilized to purify the antibody from rabbit serum. The purified antibody was obtained through an affinity purification process.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. Avoid exposing the product to repeated freeze and thaw cycles as it may compromise its stability and effectiveness. It is crucial to adhere to these storage guidelines to ensure that the product remains in optimal condition for its intended use.

Storage buffer

The rabbit IgG solution is prepared in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C. The solution is stable for 12 months starting from the date of receipt.

Target

Background

 Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.

Celluar localization

P18505