Rabbit Anti-GAD1 Polyclonal Antibody #abs130100

abs130100-50ug

1-2 Weeks

201

abs130100-100ug

1-2 Weeks

301

Description

GAD67 is an enzyme that facilitates the synthesis of GABA, and it is a member of the group II decarboxylase family. It forms a homodimer structure. Alternative splicing results in the production of three isoforms of the human GAD67 protein.

Other names

The 67 kDa glutamic acid decarboxylase, also known as GAD-67 or GAD1, is an enzyme that plays an important role in the central nervous system. It is encoded by the CPSQ1 gene and is also known as DCE1 or FLJ45882.
GAD-67 catalyzes the conversion of glutamic acid into the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) by removing a carboxyl group. This process is crucial for maintaining the balance between excitation and inhibition in the brain.
The GAD-67 isoform has a molecular weight of 67 kDa and is mainly expressed in the brain. It is essential for the normal development and function of the nervous system and is involved in various neurological disorders, such as epilepsy, schizophrenia, and Parkinson's disease.
In summary, GAD-67 is a vital enzyme that plays a critical role in neurotransmitter synthesis and neurological function. Its dysregulation can lead to various neurological disorders, making it a promising target for therapeutic intervention.

Source

Rabbit

Specificity

The detection of endogenous levels of total GAD1 is possible using the GAD1 antibody. It is important to note that this antibody specifically targets and detects all forms of GAD1 present in a sample. This provides researchers with a reliable tool to analyze and study GAD1 expression and regulation in various biological systems. By detecting and quantifying total GAD1 levels, researchers can gain valuable insights into the role and function of GAD1 in different cellular processes, as well as its potential implications in various diseases and conditions.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine;Horse;

Application

To give you the recommended dilutions for our antibody, we suggest using a dilution range of 1:500 to 1:3000 for Western Blot assays, 1:50 to 1:200 for Immunohistochemistry assays, 1:100 to 1:500 for Immunofluorescence/Immunocytochemistry assays, and 1:20000 to 1:40000 for ELISA assays using the peptide. These dilutions will ensure optimal results and accurate data for your experiments.

Immunogen

A synthesized peptide derived from human GAD1.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality, store for a year at -20 °C. Prevent freezing and thawing repeatedly. Repeating freezing and thawing cycles should be avoided.

Storage buffer

The recommended storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that the stability of Rabbit IgG is guaranteed for a period of 12 months starting from the date of receipt.

Target

Background

Catalyzes the production of GABA.

Tissue specificity

Isoform 3 is absent in the brain while being present in the pancreatic islets, testis, adrenal cortex, and potentially other endocrine tissues. The expression pattern suggests that this isoform is predominantly found in endocrine tissues rather than in neural tissues.

Celluar localization

Mitochondrion;Plasma Membrane;

UniPort

Q99259