Rabbit Anti-GABBR1 Polyclonal Antibody #abs130099

abs130099-50ug

1-2 Weeks

201

abs130099-100ug

1-2 Weeks

301

Description

The GABBR1 receptor, which responds to the neurotransmitter GABA, is closely involved in regulating inhibitory synaptic transmission. Its activity is governed by G-proteins, which act to inhibit adenylyl cyclase activity, stimulate phospholipase A2, activate potassium channels, inactivate voltage-dependent calcium channels, and modulate inositol phospholipids hydrolysis. Both pre-synaptic and post-synaptic GABA-B-R influence neurotransmitter release and neuronal excitability respectively. Furthermore, this receptor is implicated in several essential physiological processes such as hippocampal long-term potentiation, slow wave sleep, muscle relaxation, and antinociception. It is activated by compound (-)-baclofen and cgp27492, and blocked by phaclofen. GABBR1 forms a heterodimer with GABA-B-R2, and interaction with JAKMIP1 is also reported. Although isoform 1E is unable to dimerize with GABA-B-R2 due to the absence of a coiled-coil interaction, it is still involved in regulating the receptor's downstream signaling pathways.

Other names

: GABA-B 1， GABA-B-R1、GABABR1 GABBR1， γ- (GABA) 。 GABA-B 1c、Gb1 GPRC3A。GABA-B 1 GABBR1 。 GABA-B ，。。
"dJ271M21.1.1" "dJ271M21.1.2" GABBR1 。 "FLJ92613" GABBR1 。，。
GABA-B 1 。 GABA-B ，，。，、，。
，GABA-B 1 ，。。

Source

Rabbit

Specificity

The detection of endogenous levels of total GABBR1 can be achieved using the GABBR1 antibody. Generating content based on the original information while ensuring a highly similar structure suggests that the GABBR1 antibody is capable of identifying the natural amounts of GABBR1 present in an organism.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

: WB: 1:500~1:3000, IHC: 1:50~1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000。，。

Immunogen

A synthesized peptide derived from human GABBR1.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was used for peptide affinity chromatography to purify the antiserum. This process resulted in a highly purified antiserum, which was essential for its intended use.

Clonality

Polyclonal Antibody

Stability & Storage

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872316, PubMed:9872744, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10906333, PubMed:10773016, PubMed:10075644, PubMed:9872744, PubMed:24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9844003). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9844003, PubMed:9872316, PubMed:10075644, PubMed:9872744, PubMed:22660477). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen (PubMed:9844003, PubMed:9872316, PubMed:24305054).

Tissue specificity

Highly expressed in brain (PubMed:9844003, PubMed:9753614, PubMed:9872744). Weakly expressed in heart, small intestine and uterus. Isoform 1A: Mainly expressed in granular cell and molecular layer (PubMed:9844003). Isoform 1B: Mainly expressed in Purkinje cells (PubMed:9844003). Isoform 1E: Predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland (PubMed:10906333).

Celluar localization

Extracellular region or secreted;Plasma Membrane;

UniPort

Q9UBS5