Phospho-MEK2 (Thr394) Rabbit Polyclonal Antibody#abs130909

abs130909-50ug

1-2 Weeks

201

abs130909-100ug

1-2 Weeks

301

Description

MEK2 belongs to the STE7 kinase family and is classified as a dual-specificity protein kinase. It undergoes phosphorylation and activation by Raf and Mos kinases. The activation process involves the phosphorylation of a Thr and a Tyr residue in a Thr-Glu-Tyr sequence present in the activation loop of ERK2 and ERK3.

Other names

Cardiofaciocutaneous syndrome (CFC syndrome or CFC4) is a genetic disorder that affects various body systems and is caused by mutations in the MAP2K2 gene. This gene encodes for the protein called Mitogen-activated protein kinase kinase 2 (MEK2), which is involved in the MAPK/ERK signaling pathway that regulates cell growth, division, and differentiation. MEK2 is also known as MAPKK2, ERK activator kinase 2, or dual specificity mitogen-activated protein kinase kinase 2.
People with CFC syndrome typically display distinctive facial features, including a prominent forehead, widely spaced eyes, and a small chin. They also have abnormalities in their heart, skin, and hair development. In addition, many individuals with CFC syndrome have intellectual disability and delayed development.
Mutations in the MAP2K2 gene disrupt the normal function of MEK2 and lead to overactivity of the MAPK/ERK pathway. This excess signaling can affect cell growth and differentiation, leading to the characteristic features of CFC syndrome.
While there is no cure for CFC syndrome, management of the symptoms and complications is important. Treatment may involve surgery, medication, and therapy.
In summary, CFC syndrome is a genetic condition caused by mutations in the MAP2K2 gene, resulting in overactivity of the MAPK/ERK signaling pathway. It is characterized by distinctive facial features, heart and skin abnormalities, intellectual disability, and delayed development. Treatment focuses on managing the symptoms and complications of the condition.

Source

Rabbit

Specificity

The detection of endogenous levels of MEK2 is specifically achieved by the Phospho-MEK2 (Thr394) Antibody, which is designed to target and identify the phosphorylation of MEK2 at Threonine 394.

Application

WB: 1:500-1:2000, IHC: 1:50-1:200, IP, ELISA (peptide): 1:20000-1:40000. Here are the revised details, arranged differently but conveying the same information:
For Western Blotting (WB), the recommended dilution range is 1:500 to 1:2000. When performing Immunohistochemistry (IHC), it is suggested to use a dilution range of 1:50 to 1:200. For Immunoprecipitation (IP) and Enzyme-Linked Immunosorbent Assay (ELISA) using peptide-based assays, the ideal dilution range is 1:20000 to 1:40000.

A highly similar content can be generated by rearranging the original content to maintain the information about a synthesized peptide derived from human MEK2, specifically around the phosphorylation site of Threonine 394. However, it is important to note that the generated content should not follow the style of ChapGPT conversation, but rather present a completely different way of speaking based on the language model.

Properties

1mg/ml

Purification

The purified rabbit serum was utilized to produce the antibody through a process of sequential chromatography involving phospho- and non-phospho-peptide affinity columns. This process allowed for the affinity purification of the antibody, resulting in a final product that is highly specific to its intended target. The use of this technique ensures the production of a top-quality antibody with the utmost precision and accuracy.

Clonality

Polyclonal Antibody

To maintain its quality for a year, store at -20 °C. It is important to prevent the product from undergoing freeze/thaw cycles repeatedly.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at -20 °C. The stability of the product is guaranteed for 12 months from the date of receipt.

Target

Background

 Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

Celluar localization

Cytoskeleton;Cytosol;Endoplasmic reticulum;Endosome;Extracellular region or secreted;Golgi apparatus;Mitochondrion;Nucleus;Peroxisome;Plasma Membrane;

P36507