Phospho-SREBP-1 (Ser439) Rabbit Polyclonal Antibody#abs130910

abs130910-50ug

1-2 Weeks

201

abs130910-100ug

1-2 Weeks

301

Description

The protein encoded by this gene acts as a transcription factor that binds to the sterol regulatory element-1 (SRE1). The SRE1 is a decamer that flanks the low density lipoprotein receptor gene as well as some genes involved in sterol biosynthesis. Initially, this protein is synthesized as a precursor that becomes attached to both the nuclear membrane and the endoplasmic reticulum.

Other names

Class D basic helix-loop-helix protein 1, also known as bHLHd1, is a transcription factor that belongs to the Sterol Regulatory Element Binding Transcription Factor 1/Protein 1 family. Its gene is labeled D630008H06 and it is associated with the regulation of lipid metabolism.
Processed Sterol Regulatory Element-Binding Protein 1, also known as SRBP1_HUMAN or SREBP1, is another name for this transcription factor. Other aliases for this protein include SREBF 1, SREBF1, SREBP 1, SREBP-1, and SREBP1c.
This protein plays an essential role in the transcriptional regulation of genes involved in the biosynthesis and uptake of cholesterol, fatty acids, and triglycerides. It binds to sterol regulatory elements (SREs) in the promoter regions of target genes, activating their transcription. In addition to its role in lipid metabolism, SREBP1 also plays a role in glucose homeostasis and insulin signaling.
Overall, SREBP1 is a crucial factor in maintaining metabolic homeostasis and dysregulation of its activity has been implicated in numerous diseases, including cardiovascular disease, diabetes, and cancer.

Source

Rabbit

Specificity

The Phospho-SREBP-1 (Ser439) Antibody specifically targets the phosphorylated form of SREBP-1 at Serine 439, allowing for the detection of endogenous levels of this protein. Through its specificity for the phosphorylated form, this antibody enables the examination of phosphorylation events specifically at Serine 439 of SREBP-1.

Predictive reaction species

Pig;Sheep;Dog;Horse;

Application

The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA(peptide) are 1:20000-1:40000. Please note that these dilution ratios are based on the original information provided and should be followed accordingly.

A peptide synthesized from the phosphorylation site of Serine 439 in human SREBP-1 has been developed. The synthetic peptide closely mimics the original sequence of human SREBP-1 and is designed to target the specific phosphorylation site at Serine 439. This novel peptide holds great potential for further research and exploration in understanding the functional implications of phosphorylation at this specific site in SREBP-1. The development of this synthesized peptide opens up new avenues for investigating the regulatory mechanisms and signaling pathways associated with SREBP-1 phosphorylation at Serine 439, ultimately leading to a better understanding of its role in cellular processes and potential therapeutic interventions.

Properties

1mg/ml

Purification

，。

Clonality

Polyclonal Antibody

To maintain quality, store the product at a temperature of -20 °C for a duration of one year. It is important to prevent the product from undergoing multiple freeze/thaw cycles.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should have a concentration of 150mM NaCl and contain 0.02% sodium azide and 50% glycerol. This product remains stable for a period of 12 months from the date of receipt.

Target

Background

 Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

Tissue specificity

Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Celluar localization

Cytosol;Endoplasmic reticulum;Golgi apparatus;Nucleus;

P36956